Spreading of Fibroblasts in Medium containing Cytochalasin B: Formation of Lamellar Cytoplasm as a Combination of Several Functionally Different Processes

Normal cultured mouse fibroblasts spreading on solid substrate extend and attach numerous pseudopods; lamellar cytoplasm is eventually formed from the attached pseudopods. Fibroblasts spreading in the presence of cytochalasin B (CB) form de novo a system of arbor-like branched processes rather than...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-01, Vol.77 (10), p.5919-5922
Main Authors: Bliokh, Z. L., Domnina, L. V., O. Yu. Ivanova, O. Yu. Pletjushkina, Svitkina, T. M., Smolyaninov, V. A., Vasiliev, J. M., Gelfand, I. M.
Format: Article
Language:English
Subjects:
B lymphocytes
Cells
Cytochalasins
Cytoplasm
Delta cells
Fibroblasts
Microfilaments
Neurons
Receptors
Seeding
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Summary:Normal cultured mouse fibroblasts spreading on solid substrate extend and attach numerous pseudopods; lamellar cytoplasm is eventually formed from the attached pseudopods. Fibroblasts spreading in the presence of cytochalasin B (CB) form de novo a system of arbor-like branched processes rather than lamellar cytoplasm. The growing and fully formed arbor-like processes, in contrast to normal lamellar cytoplasm, have low contractility and are unable to clear patched concanavalin A receptors from their surfaces; their attachment sites are not associated with microfilament bundles. The cells spreading in medium containing CB and Colcemid do not form well-organized branched structures but extend and attach numerous unstable pseudopods. It is suggested that normal formation of lamellar cytoplasm can be regarded as a combination of several functionally different processes: (a) of rudimentary pseudopodial reactions resistant to CB and Colcemid; (b) of CB-sensitive lamellization; and (c) of Colcemidsensitive stabilization.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.10.5919