Lack of Influence of the Epidermis on Underlying Cell Development in Leaflets of Pisum sativum var. argenteum (Fabaceae)

We explored whether epidermal pressure regulates cell and organ growth in leaflets of Pisum sativum var. argenteum, a mutant cultivar of the garden pea characterized by reduced adhesion between the epidermis and subjacent mesophyll. Developing leaflets of leaves arising at three positions on the see...

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Bibliographic Details
Published in:Annals of botany 1999-01, Vol.83 (1), p.1-10
Main Authors: WILSON, MARY K. P., BRUCK, DAVID K.
Format: Article
Language:English
Subjects:
Cell division
Cell growth
Epidermal cells
Epidermis
Humidity
Mesophyll
Mesophyll cells
Organogenesis
Peas
Plants
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Summary:We explored whether epidermal pressure regulates cell and organ growth in leaflets of Pisum sativum var. argenteum, a mutant cultivar of the garden pea characterized by reduced adhesion between the epidermis and subjacent mesophyll. Developing leaflets of leaves arising at three positions on the seedling axis were peeled in situ and grown to maturity in humidity chambers. The mature anatomy and morphology could be accurately assessed because wound responses normally associated with peeling were prevented by the Arg mutation that permitted peeling without damage to the mesophyll and by the humidity chambers that protected peeled areas from desiccation. The mesophyll cell size, state of differentiation, and layering pattern as well as the overall morphology of mature, peeled leaflets were indistinguishable from those of mature, intact leaflets grown under the same conditions. The epidermis exerted no detectable regulatory effect on the expansion of the leaflets as a whole or on the tissue layers and cells within the leaflets.
ISSN:0305-7364
1095-8290
DOI:10.1006/anbo.1998.0773