Assembly and movement of a plant virus carrying a green fluorescent protein overcoat

Potato virus X (PVX) is a filamentous plant virus infecting many members of the family Solanaceae. A modified form of PVX, PVX.GFP-CP which expressed a chimeric gene encoding a fusion between the 27-kDa Aequorea victoria green fluorescent protein and the amino terminus of the 25-kDa PVX coat protein...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-06, Vol.93 (13), p.6286-6290
Main Authors: Santa Cruz, S. (Scottish Crop Research Institute, Dundee, United Kingdom.), Chapman, S, Roberts, A.G, Prior, D.A.M
Format: Article
Language:English
Subjects:
ADN
Aequorea victoria
Amino Acid Sequence
Antiserum
Botany
Capsid - genetics
Cell aggregates
Epidermal cells
EXPERIMENTACION IN VIVO
EXPERIMENTATION IN VIVO
Flowers & plants
FLUORESCENCE
FLUORESCENCIA
GENE
GENES
GENIE BIOCHIMIQUE
GENIE GENETIQUE
Green Fluorescent Proteins
INFECCION EXPERIMENTAL
INFECTION EXPERIMENTALE
INGENIERIA BIOQUIMICA
INGENIERIA GENETICA
Leaves
Luminescent Proteins - genetics
Mesophyll cells
Microscopy, Confocal
Microscopy, Electron
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - virology
NICOTIANA TABACUM
PATHOGENESE
PATOGENESIS
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified - genetics
Plants, Genetically Modified - virology
Plants, Toxic
Plasmids
potato virus X
Potexvirus - genetics
Potexvirus - physiology
POTEXVIRUS X DE LA PATATA
POTEXVIRUS X POMME DE TERRE
Protein Processing, Post-Translational
PROTEINAS
PROTEINE
Proteins
RNA, Messenger - genetics
Virion
Virions
Virus Assembly
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
Viruses
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Description
Summary:Potato virus X (PVX) is a filamentous plant virus infecting many members of the family Solanaceae. A modified form of PVX, PVX.GFP-CP which expressed a chimeric gene encoding a fusion between the 27-kDa Aequorea victoria green fluorescent protein and the amino terminus of the 25-kDa PVX coat protein, assembled into virions and moved both locally and systemically. The PVX.GFP-CP virions were over twice the diameter of wild-type PVX virions. Assembly of PVX.GFP-CP virions required the presence of free coat protein subunits in addition to the fusion protein subunits. PVX.GFP-CP virions accumulated as paracrystalline arrays in infected cells similar to those seen in cells infected with wild-type PVX. The formation of virions carrying large superficial fusions illustrates a novel approach for production of high levels of foreign proteins in plants. Aggregates of PVX.GFP-CP particles were fluorescent, emitting green light when excited with ultraviolet light and could be imaged using confocal laser scanning microscopy. The detection of virus particles in infected tissue demonstrates the potential of fusions between the green fluorescent protein and virus coat protein for the non-invasive study of virus multiplication and spread
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.13.6286