Cancer Antigens are Expressed in a Carcinogen-Transformed Bloom Syndrome B-Lymphoblastoid Cell Line

We have cloned malignant cells carrying specific antigens associated with ovarian cancer (OVC) and malignant lymphoma (ML) from BS-SHI-4M cells, a line derived from a 1-methyl-3-nitro-1-nitrosoguanidine-treated B-lymphoblastoid cell line isolated from a patient with Bloom syndrome. Since BS-SHI-4M c...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-11, Vol.85 (21), p.8211-8215
Main Authors: Shiraishi, Yukimasa, Soma, Hiroaki
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
ANIMAL CELLS
Antibodies
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
Antigens, Neoplasm - biosynthesis
Antigens, Neoplasm - genetics
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
Bloom syndrome
Bloom Syndrome - genetics
Bloom Syndrome - immunology
CARCINOGENESIS
CARCINOGENS
Cell Line
Cell lines
CELL TRANSFORMATIONS
Cells
Cellular immunity
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
Clone Cells - analysis
DISEASES
DNA, Neoplasm - analysis
ETIOLOGY
Female
Fluorescent antibody techniques
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genetic Markers
HEREDITARY DISEASES
HETEROCHROMOSOMES
Humans
IMMUNE SYSTEM DISEASES
Indirect fluorescent antibody technique
KARYOTYPE
Karyotyping
Lymphoma - genetics
Lymphoma - metabolism
LYMPHOMAS
Methylnitronitrosoguanidine
MUTATIONS
NEOPLASMS
NITROSO COMPOUNDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
Ovarian Neoplasms - genetics
Ovarian Neoplasms - metabolism
PATHOGENESIS
PATIENTS
Tissue, organ and graft immunology
TUMOR CELLS
Y CHROMOSOME
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Description
Summary:We have cloned malignant cells carrying specific antigens associated with ovarian cancer (OVC) and malignant lymphoma (ML) from BS-SHI-4M cells, a line derived from a 1-methyl-3-nitro-1-nitrosoguanidine-treated B-lymphoblastoid cell line isolated from a patient with Bloom syndrome. Since BS-SHI-4M cells react with sera from various individual cancer patients at relatively low frequencies (2-9%), as detected by an indirect immunofluorescence technique, cell clones that specifically react with sera from patients with OVC and ML were separated by the ``panning'' method in which polystyrene dishes were coated with sera from OVC and ML patients and cells with the corresponding antigens bound to the dishes. Subsequent cloning by limiting dilution provided cell clones highly enriched for OVC- and ML-associated antigens. Karyotype analyses revealed that cell clones with OVC and ML antigens had common marker chromosomes, der(14)t(14;14) (p11;q11),t(6;?)(p25;?) and t(9;?)(q34;?), besides t(17;?) (q25;?) found in the OVC-antigen-positive clones and t(5;?) (p13;?),t(7;?)(q36;?) found in the ML-antigen-positive clones. Interestingly, in cell clones with a strong OVC antigen response, the distal part of the Y chromosome (Yq11) was missing in 100% of the cells. Therefore the cell line BS-SHI-4M appears to be a reservoir of cell clones each of which carries a specific tumor antigen and thus provides a potential tool for rapid serological diagnosis of cancer.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.21.8211