Analysis of the antioxidant response ofNicotiana benthamianato infection with two strains ofPepper mild mottle virus

The present study was carried out to investigate the role of reactive oxygen species (ROS) metabolism in symptom development and pathogenesis inNicotiana benthamianaplants upon infection with two strains ofPepper mild mottle virus, the Italian (PMMoV-I) and the Spanish (PMMoV-S) strains. In this hos...

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Bibliographic Details
Published in:Journal of experimental botany 2012-01, Vol.63 (15), p.5487-5496
Main Authors: Hakmaoui, A., Pérez-Bueno, M. L., García-Fontana, B., Camejo, D., Jiménez, A., Sevilla, F., Barón, M.
Format: Article
Language:English
Subjects:
Antioxidants
Chloroplasts
Enzymes
Infections
Leaves
Oxidative stress
Physiology
Plant physiology
Plants
RESEARCH PAPER
Viruses
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Summary:The present study was carried out to investigate the role of reactive oxygen species (ROS) metabolism in symptom development and pathogenesis inNicotiana benthamianaplants upon infection with two strains ofPepper mild mottle virus, the Italian (PMMoV-I) and the Spanish (PMMoV-S) strains. In this host, it has been shown that PMMoV-I is less virulent and plants show the capability to recover 21 d after inoculation. Analyses of oxidative stress biomarkers, ROS-scavenging enzyme activities, and antioxidant compounds were conducted in plants at different post-infection times. Only PMMoV-I stimulated a defence response through: (i) up-regulation of different superoxide dismutase isozymes; (ii) maintenance of adequate levels of three peroxiredoxins (2-Cys Prx, Prx IIC, and Prx IIF); and (iii) adjustments in the glutathione pool to maintain the total glutathione content. Moreover, there was an increase in the level of oxidized glutathione and ascorbate in the recovery phase of PMMoV-I-infected plants. The antioxidant response and the extent of oxidative stress inN. benthamianaplants correlates to: (i) the severity of the symptoms elicited by either strain of PMMoV; and (ii) the high capacity of PMMoV-I-infected plants for symptom recovery and delayed senescence, compared with PMMoV-S-infected plants.
ISSN:0022-0957
1460-2431