Steric confinement of proteins on lipid membranes can drive curvature and tubulation

Deformation of lipid membranes into curved structures such as buds and tubules is essential to many cellular structures including endocytic pits and filopodia. Binding of specific proteins to lipid membranes has been shown to promote membrane bending during endocytosis and transport vesicle formatio...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2010-04, Vol.107 (17), p.7781-7786
Main Authors: Stachowiak, Jeanne C., Hayden, Carl C., Sasaki, Darryl Y., Whitesides, George M.
Format: Article
Language:English
Subjects:
Bending
Binding sites
Biological Sciences
Biophysics
Cell membranes
Curvature
Fluorescence
Histidine - metabolism
Imino acids
Lipid bilayers
Lipids
Membranes
Models, Chemical
Molecular weight
P branes
Phosphatidylcholines - chemistry
Phosphatidylcholines - metabolism
Protein Binding - physiology
Proteins
Proteins - metabolism
Solubility
Unilamellar Liposomes - metabolism
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Summary:Deformation of lipid membranes into curved structures such as buds and tubules is essential to many cellular structures including endocytic pits and filopodia. Binding of specific proteins to lipid membranes has been shown to promote membrane bending during endocytosis and transport vesicle formation. Additionally, specific lipid species are found to colocalize with many curved membrane structures, inspiring ongoing exploration of a variety of roles for lipid domains in membrane bending. However, the specific mechanisms by which lipids and proteins collaborate to induce curvature remain unknown. Here we demonstrate a new mechanism for induction and amplification of lipid membrane curvature that relies on steric confinement of protein binding on membrane surfaces. Using giant lipid vesicles that contain domains with high affinity for his-tagged proteins, we show that protein crowding on lipid domain surfaces creates a protein layer that buckles outward, spontaneously bending the domain into stable buds and tubules. In contrast to previously described bending mechanisms relying on local steric interactions between proteins and lipids (i.e. helix insertion into membranes), this mechanism produces tubules whose dimensions are defined by global parameters: domain size and membrane tension. Our results suggest the intriguing possibility that confining structures, such as lipid domains and protein lattices, can amplify membrane bending by concentrating the steric interactions between bound proteins. This observation highlights a fundamental physical mechanism for initiation and control of membrane bending that may help explain how lipids and proteins collaborate to create the highly curved structures observed in vivo.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0913306107