The Partition Coefficient of14C2H4in Plant Tissue as a Screening Test for Metabolism or Compartmentation of Ethylene

A method is described for the direct measurement of14C2H4in toluene scintillator and for the measurement of toluene-soluble14C accumulated in tissue during14C2H4(0.7-1.0 μ l/l) treatment. Using these methods the partition coefficient for ethylene between air and plant tissues was estimated. Tissues...

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Bibliographic Details
Published in:The New phytologist 1978-11, Vol.81 (3), p.499-504
Main Authors: Jerie, P. H., Shaari, A. R., Zeroni, M., Hall, M. A.
Format: Article
Language:English
Subjects:
Coefficients
Hall effect
Plant tissues
Quaternary ammonium compounds
Radiocarbon
Scintillation counters
Scintillation counting
Screening tests
Seeds
Tissue samples
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Summary:A method is described for the direct measurement of14C2H4in toluene scintillator and for the measurement of toluene-soluble14C accumulated in tissue during14C2H4(0.7-1.0 μ l/l) treatment. Using these methods the partition coefficient for ethylene between air and plant tissues was estimated. Tissues from a substantial proportion of 36 species tested gave partition coefficients significantly higher than that for water (0.11) indicating either the sequestration of14C2H4or the accumulation of toluene-soluble metabolites. Steam-killed tissue gave partition coefficients similar to that of water.
ISSN:0028-646X
1469-8137