Purification and properties of the Mboll, a class-IIs restriction endonuclease

After five purification steps a homogeneous preparation of endonuclease Mboll was obtained, and several properties of the enzyme were determined. Mboll is a monomer, with Mr under native and denaturing conditions being 47 − 49 × 103 Da. Endonuclease Mboll is a basic protein (pl 8.3) which remains ac...

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Bibliographic Details
Published in:Nucleic acids research 1992-02, Vol.20 (3), p.433-438
Main Authors: Sektas, Marian, Kaczorowski, Tadeusz, Podhajska, Anna J.
Format: Article
Language:English
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Summary:After five purification steps a homogeneous preparation of endonuclease Mboll was obtained, and several properties of the enzyme were determined. Mboll is a monomer, with Mr under native and denaturing conditions being 47 − 49 × 103 Da. Endonuclease Mboll is a basic protein (pl 8.3) which remains active when Mg2+ is replaced by Mn2+, Co2+, Ca2+, or Fe2+. Mboll exhibits a star activity in the presence of some of the following reagents or ions: DMSO, glycerol, ethanol (and Co2+ or Mn2+ at pH 6). MboII does not bend DNA and is heat sensitive, losing activity after 15 min at 50°C.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.3.433