Interleukin-8 Production by Human Peritoneal Mesothelial Cells in Response to Tumor Necrosis Factor-α, Interleukln-1, and Medium Conditioned by Macrophages Cocultured with Staphylococcus epidermidis

Patients treated with continuous ambulatory peritoneal dialysis (CAPD) may suffer from recurrent peritonitis episodes caused by Staphylococcus epidermidis. Early recruitment of granulocytes from the peripheral blood is important for the peritoneal antibacterial defense of CAPD patients. In this stud...

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Bibliographic Details
Published in:The Journal of infectious diseases 1993-11, Vol.168 (5), p.1202-1210
Main Authors: Betjes, Michiel G. H., Tuk, Cornelis W., Struijk, Dirk G., Krediet, Raymond T., Arisz, Lambertus, Hart, Margreet, Beelen, Rob H. J.
Format: Article
Language:English
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Summary:Patients treated with continuous ambulatory peritoneal dialysis (CAPD) may suffer from recurrent peritonitis episodes caused by Staphylococcus epidermidis. Early recruitment of granulocytes from the peripheral blood is important for the peritoneal antibacterial defense of CAPD patients. In this study, human peritoneal mesothelial cells were shown to produce high levels of interleukin-8 (IL-8) in response to IL-lβ and tumor necrosis factor-α (TNFα) but not lipopolysaccharide or S. epidermidis. Coculture of peritoneal macrophages with S. epidermidis induced high levels of IL-1α, IL-1β, and TNFα in 24-h-conditioned medium. Preincubation of this medium with anti-TNFα, anti-IL-1α, or anti-IL-1β partially blocked stimulation of IL-8 production by mesothelial cells. Added together, these antibodies abolished IL-8 production to a level just above background. Migration of granulocytes to the stimulated mesothelial cell-conditioned medium could be totally blocked with rabbit polyclonal anti-IL-8 antibody. Thus, mesothelial cells are important for the recruitment of granulocytes into the peritoneal cavity.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/168.5.1202