Rapid and delayed effects of single-walled carbon nanotubes in glioma cells

Single-walled carbon nanotubes (SWCNTs) demonstrate a strong potential as an optically activated theranostic nano-agent. However, using SWCNTs in theranostics still requires revealing mechanisms of the SWCNT-mediated effects on cellular functions. Even though rapid and delayed cellular responses can...

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Bibliographic Details
Published in:Nanotechnology 2021-12, Vol.32 (50), p.505103
Main Authors: Golubewa, Lena, Kulahava, Tatsiana, Timoshchenko, Igor, Shuba, Mikhail, Svirko, Yuri, Kuzhir, Polina
Format: Article
Language:English
Subjects:
Animals
autophagy
Cell Line, Tumor
Cell Proliferation - drug effects
Cytoskeleton - drug effects
DNA - chemistry
Endocytosis
Glioma - metabolism
Glioma - pathology
Membrane Potential, Mitochondrial - drug effects
Mitochondria - metabolism
Mitochondria - pathology
mitochondrial membrane potential
Nanotubes, Carbon - chemistry
Nanotubes, Carbon - toxicity
Rats
single-walled carbon nanotubes
superoxide
Superoxides - metabolism
Theranostic Nanomedicine
theranostics
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Summary:Single-walled carbon nanotubes (SWCNTs) demonstrate a strong potential as an optically activated theranostic nano-agent. However, using SWCNTs in theranostics still requires revealing mechanisms of the SWCNT-mediated effects on cellular functions. Even though rapid and delayed cellular responses can differ significantly and may lead to undesirable consequences, understanding of these mechanisms is still incomplete. We demonstrate that introducing short (150-250 nm) SWCNTs into C6 rat glioma cells leads to SWCNT-driven effects that show pronounced time dependence. Accumulation of SWCNTs is carried out due to endocytosis with modification of the actin cytoskeleton but not accompanied with autophagy. Its initial stage launches a rapid cellular response via significantly heightened mitochondrial membrane potential and superoxide anion radical production, satisfying the cell demand of energy for SWCNT transfer inside the cytoplasm. In the long term, SWCNTs agglomerate to micron-sized structures surrounded by highly active mitochondria having parameters return to control values. SWCNTs postponed effects are also manifested themselves in the suppression of the cell proliferative activity with further restoration after five passages. These results demonstrate relative cellular inertness and safety of SWCNTs eliminating possible side effects caused by optically activated theranostic applications.
ISSN:0957-4484
1361-6528
DOI:10.1088/1361-6528/ac28da