Interaction of alcohol and kola nut on brain sodium pump activity in Wistar rats

The effect of interaction of alcohol and kola nut on sodium pump activity was studied in Wistar rats. Thirty Wistar rats were divided into six groups of five rats per group. Control received a placebo (4 mL of distilled water). Groups 2-6 were treated for a period of 21 days with 10% (v/v) alcohol,...

Full description

Saved in:

Bibliographic Details
Published in:	Toxicological and environmental chemistry 2009-03, Vol.91 (2), p.357-362
Main Authors:	Obochi, G.O., Abara, A.E., Malu, S.P., Eteng, M.U., Umoh, I.B.
Format:	Article
Language:	eng
Subjects:	alcohol ATPase caffeine enzyme kola nut
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The effect of interaction of alcohol and kola nut on sodium pump activity was studied in Wistar rats. Thirty Wistar rats were divided into six groups of five rats per group. Control received a placebo (4 mL of distilled water). Groups 2-6 were treated for a period of 21 days with 10% (v/v) alcohol, 50 mg caffeine/kg, 50 mg kola nut/kg, or a combination of 10% (v/v) alcohol + 50 mg kola nut/kg, or 10% (v/v) alcohol + 50 mg caffeine/kg, respectively. One day after the final exposure, brains were harvested and several biochemical parameters examined including activities of total ATPase, ouabain-insensitive ATPase, ouabain sensitive ATPase (Na + -K + -ATPase) and levels of nonenzymatic breakdown of ATP and inorganic phosphate (P i ) released. Results showed that the essential enzyme of the brain responsible for neuronal function, Na + -K + -ATPase, was inhibited by alcohol-kola nut co-administration relative to control, resulting in a decreased ATP production, ion transport and action potential, leading to loss of neuronal activities.
ISSN:	0277-2248 1029-0486