Two-photon fluorescent labels with enhanced sensitivity for biological imaging

The development of two-photon laser scanning fluorescence microscopy (TPLSM) has provided a new capability for 3D imaging in whole and living tissues, reduced photobleaching effects, and greater depth penetration. Since the rate of two-photon excitation is proportional to the square of the light int...

Full description

Saved in:
Bibliographic Details
Main Authors: Wenseleers, W., Stellacci, F., Pond, S., Parker, T., Mangel, T., Halik, M., Meyer-Friedrichsen, T., Perry, J.W., Marder, S.R., Heikal, A.A., Shaohui Huang, Webb, W.W.
Format: Conference Proceeding
Language:English
Subjects:
Absorption
Biological systems
Biomedical optical imaging
Charge transfer
Diagnosis
Diseases
Fluorescence
Laser beams
Laser excitation
Microscopic examination
Microscopy
Optical imaging
Optical sensors
Quantum efficiency
Solvents
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The development of two-photon laser scanning fluorescence microscopy (TPLSM) has provided a new capability for 3D imaging in whole and living tissues, reduced photobleaching effects, and greater depth penetration. Since the rate of two-photon excitation is proportional to the square of the light intensity, degree of excitation falls off roughly as the fourth power of distance from the focal plane. The full potential of two-photon fluorescence microscopy has not been realized, in part, because fluorophores under use have been developed for single photon excitation and have not been optimized for two-photon absorption. Highly efficient two-photon fluorophores are needed to allow sensitive detection of small numbers of labeled sites and to reduce the optical power of the exciting laser beam that is needed to produce adequate signal levels. The sensitivity of a two-photon fluorescence label is determined by the product of the two-photon absorption cross section and the fluorescence quantum efficiency.
ISSN:1092-8081
2766-1733
DOI:10.1109/LEOS.2000.890812