The Primary Mechanism of Photoexcited TiO2 Nanoparticles-induced Apoptosis in Human Hepatoma Bel-7402 Cells

To investigate the primary mechanism of photoexcited TiO 2 nanoparticles -induced apoptosis in Bel-7402 cell. Bel-7402 cells were treated with photoexcited TiO 2 nanoparticles. Cells inhibitory rate was assayed by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) method. Cell...

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Bibliographic Details
Main Authors: Xia, Chun-Hui, Yu, Wen-Xue, Wang, Bai-Qi, Wang, Yu, Wang, Lu
Format: Conference Proceeding
Language:English
Subjects:
apoptosis
bcl-2
Biomedical engineering
Biomedical informatics
Cancer
caspase-3
Cells (biology)
Chemistry
Educational institutions
Helium
Humans
Materials science and technology
Nanoparticles
photoexcited TiO2 nanoparticles
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Summary:To investigate the primary mechanism of photoexcited TiO 2 nanoparticles -induced apoptosis in Bel-7402 cell. Bel-7402 cells were treated with photoexcited TiO 2 nanoparticles. Cells inhibitory rate was assayed by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) method. Cell apoptosis was evaluated by both flow cytometry (FCM) and Haematoxylin and Eosin (HE) Staining method. Expression of caspase 3 and bcl-2 were determined by Western blot, respectively. Photoexcited TiO 2 nanoparticles exhibited good inhibition effects on Bel- 7402 cells under appropriate TiO 2 concentration; Both morphological observations and FCM results showed that photoexcited TiO 2 nanoparticles induced apoptosis in Bel-7402 cells with concomitant increase of activated caspase-3 expression and decrease of bcl-2 expression. Photoexcited TiO 2 nanoparticles can apparently inhibit Bel-7402 cells under appropriate concentration, and the inhibition process follows first order reaction rule approximately. In addition, photoexcited TiO 2 nanoparticles can remarkably induce apoptosis in Bel-7402 cells, and the molecular mechanism of apoptosis is closely associated with increase of activated caspase-3 and down-regulation of the bcl-2 expression. The present work provides strong experimental foundation of Photoexcited TiO 2 nanoparticles in the clinical treatment of tumor.
ISSN:1948-2914
1948-2922
DOI:10.1109/BMEI.2008.158