Optimization of Gene Transfection Condition using Taguchi Method for an Electroporation Microchip

This study has demonstrated the optimization of an electroporation (EP) system for gene transfection by using the Taguchi method. The parameters of the EP system, which could have an influence on the transecting rate, were optimized, resulting in values of 50 μm electrode gap, 80 μg/mL pEGFP-Nl conc...

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Bibliographic Details
Main Authors: Keng-Shiang Huang, Sheng-Chung Yang, Hung-Yi Chen, Yu-Cheng Lin
Format: Conference Proceeding
Language:English
Subjects:
Biomembranes
Cells (biology)
DNA
Electrodes
electroporation
gene transfection
Glass
Nonuniform electric fields
Optimization methods
Research and development
Substrates
Taguchi method
Voltage
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Summary:This study has demonstrated the optimization of an electroporation (EP) system for gene transfection by using the Taguchi method. The parameters of the EP system, which could have an influence on the transecting rate, were optimized, resulting in values of 50 μm electrode gap, 80 μg/mL pEGFP-Nl concentration, 6 V pulse voltage, and 2-pulse. A larger-the-better Ω transformation was employed for better additivity. The analyzed results of interaction A × C, A × D, and C × D showed that there were strong interactions on level 3 of factors A, C, and D to each others. Using optimized EP system, the pEGFP-Nl plasmid was successfully transfected into the basal cell carcinoma cell line (BCC) with 40.36% average transecting rate.
ISSN:2159-547X
DOI:10.1109/SENSOR.2007.4300263