Bovine mammary gene expression profiling using a cDNA microarray enhanced for mammary-specific transcripts

1 Department of Animal Science 2 Center for Animal Functional Genomics 3 Genomics Technology Support Facility, Michigan State University, East Lansing, Michigan 48824 A cDNA microarray resource enhanced for transcripts specific to the bovine mammary gland (BMAM) has been developed and used in pilot...

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Bibliographic Details
Published in:Physiological genomics 2003-12, Vol.16 (1), p.8-18
Main Authors: Suchyta, Steven P, Sipkovsky, Sue, Halgren, Robert G, Kruska, Rachael, Elftman, Michael, Weber-Nielsen, Miriam, Vandehaar, Michael J, Xiao, Lan, Tempelman, Robert J, Coussens, Paul M
Format: Article
Language:English
Subjects:
Animals
Cattle
DNA, Complementary - genetics
Expressed Sequence Tags
Gene Expression Profiling
Gene Expression Regulation
Humans
Lactation - genetics
Mammary Glands, Animal - growth & development
Mammary Glands, Animal - metabolism
Mammary Glands, Animal - physiology
Mammary Neoplasms, Animal - genetics
Oligonucleotide Array Sequence Analysis
Organ Specificity
Pilot Projects
Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - genetics
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Summary:1 Department of Animal Science 2 Center for Animal Functional Genomics 3 Genomics Technology Support Facility, Michigan State University, East Lansing, Michigan 48824 A cDNA microarray resource enhanced for transcripts specific to the bovine mammary gland (BMAM) has been developed and used in pilot studies to examine gene expression profiles in the mammary gland. One goal driving development of this resource was to shed some light on the pathways and mechanisms specifically related to bovine mammary gland growth and development. To accomplish this, gene expression patterns from bovine adipose, liver, adrenal, lymph, spleen, thymus, gut, and developing mammary tissue were compared using the BMAM microarray. We have thus identified a putative set of 16 genes being preferentially expressed in developing mammary gland. Another of our long-term goals is to elucidate the genes and pathways associated with bovine lactation and involution and to use these as a model for human mammary gland development as it relates to human breast cancer risks. To begin this process, we conducted a pilot study, comparing gene expression profiles of lactating bovine mammary tissue against nonlactating tissue on the BMAM microarray. Our results have yielded many novel and interesting genes exhibiting differential expression in lactating mammary tissue, including oncogenes (VAV3, C-myc), mediators of apoptosis (Caspase 8), and cell cycle regulators (LASP1). mammary development; lactation; expressed sequence tag
ISSN:1094-8341
1531-2267
DOI:10.1152/physiolgenomics.00028.2003