Morphological and functional recovery from diaphragm injury: an in vivo rat diaphragm injury model

Department of Medicine, University of Montreal, Montreal, Quebec H2L 4M1; and Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada H2X 2P2 Our objective was to develop an in vivo model to study the timing and mechanisms underlying diaphragm injury and repair. Diaphragm injury w...

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Bibliographic Details
Published in:Journal of applied physiology (1985) 2001-06, Vol.90 (6), p.2269-2278
Main Authors: Hayot, M, Barreiro, E, Perez, A, Czaika, G, Comtois, A. S, Grassino, A. E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Caffeine
Caffeine - toxicity
Coloring Agents
Diaphragm - injuries
Diaphragm - pathology
Diaphragm - ultrastructure
Indicators and Reagents
Injuries
Medical sciences
Membranes
Membranes - pathology
Microscopy, Fluorescence
Muscle Contraction - physiology
Muscular system
Phosphodiesterase Inhibitors - toxicity
Pneumology
Rats
Respiratory system
Respiratory system : syndromes and miscellaneous diseases
Solutions
Time Factors
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Summary:Department of Medicine, University of Montreal, Montreal, Quebec H2L 4M1; and Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada H2X 2P2 Our objective was to develop an in vivo model to study the timing and mechanisms underlying diaphragm injury and repair. Diaphragm injury was induced in anesthetized rats by the application of a 100 mM caffeine solution for a 10-min period to the right abdominal diaphragm surface. Diaphragms were removed 1, 4, 6, 12, 24, 48,   72, and 96 h and 10 days after the injury, with contractile function being assessed in strips in vitro by force-frequency curves. The extent of caffeine-induced membrane injury was indicated by the percentage of fibers with a fluorescent cytoplasm revealed by inward leakage of the procion orange dye. One hour after caffeine exposure, 32.9 ± 3.1 (SE) % of fibers showed membrane injury that resulted in 70% loss of muscle force. Within 72-96 h, the percentage of fluorescent cells decreased to control values. Muscle force, however, was still reduced by 30%. Complete muscle strength recovery was observed 10 days after the injury. Whereas diaphragmatic fiber repair occurred within 4 days after injury induction, force recovery took up to 10 days. We suggest that the caffeine-damaged rat diaphragm is a useful model to study the timing and mechanisms of muscle injury and repair. respiratory muscles; membrane damage; caffeine; remodeling
ISSN:8750-7587
1522-1601
DOI:10.1152/jappl.2001.90.6.2269