Analysis of the afferent limb of the vesicovascular reflex using neurotoxins, resiniferatoxin and capsaicin

Departments of Pharmacology and Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261; Division of Urology, Chang Gung Memorial Hospital, Kaohsiung 833; and Department of Urology, National Yang Ming University, School of Medicine, Taipei 155, Taiwan The afferent limb o...

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Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2001-10, Vol.281 (4), p.1302-R1310
Main Authors: Chuang, Yao-Chi, Fraser, Matthew O, Yu, Yongbei, Beckel, Jonathan M, Seki, Satoshi, Nakanishi, Yasukazu, Yokoyama, Hitoshi, Chancellor, Michael B, Yoshimura, Naoki, de Groat, William C
Format: Article
Language:English
Subjects:
Administration, Intravesical
Afferent Pathways - drug effects
Afferent Pathways - physiology
Animals
Arteries - drug effects
Arteries - physiology
Blood Pressure - drug effects
Blood Pressure - physiology
Capsaicin - administration & dosage
Dilatation
Diterpenes - administration & dosage
Female
Injections, Subcutaneous
Muscle Contraction - physiology
Nerve Fibers - drug effects
Nerve Fibers - physiology
Neurotoxins - pharmacology
Rats
Rats, Sprague-Dawley
Reflex - drug effects
Reflex - physiology
Urinary Bladder - innervation
Urinary Bladder - physiology
Urination - drug effects
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Summary:Departments of Pharmacology and Urology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261; Division of Urology, Chang Gung Memorial Hospital, Kaohsiung 833; and Department of Urology, National Yang Ming University, School of Medicine, Taipei 155, Taiwan The afferent limb of the vesicovascular reflex (VV-R) evoked by distension or contraction of the urinary bladder (UB) was studied in urethane-anesthetized female rats by examining the changes in VV-R after administration of C-fiber afferent neurotoxins [capsaicin and resiniferatoxin (RTX)]. Systemic arterial blood pressure increased parallel (5.1 to 53.7 mmHg) with graded increases in UB pressure (20 to 80 cmH 2 O) or during UB contractions. The arterial pressor response to UB distension was significantly reduced (60-85%) by acute or chronic (4 days earlier) intravesical administration of RTX (100-1,000 nM) or by capsaicin (125 mg/kg sc) pretreatment (4 days earlier). Chronic neurotoxin treatments also increased the volume threshold (>100%) for eliciting micturition in anesthetized rats but did not change voiding pressure. Acute RTX treatment (10-50 nM) did not alter the arterial pressor response during reflex UB contractions, whereas higher concentrations of RTX (100-1,000 nM) blocked reflex bladder contractions. It is concluded that VV-R is triggered primarily by distension- and contraction-sensitive C-fiber afferents located, respectively, near the luminal surface and deeper in the muscle layers of the bladder. urinary bladder; afferent nerve
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.2001.281.4.r1302