Sepsis is associated with increased ubiquitinconjugating enzyme E214k mRNA in skeletal muscle

Department of Surgery, University of Cincinnati, Cincinnati, Ohio 45267; Shriners Burns Institute, Cincinnati, Ohio 45229; and ProScript, Incorporated, Cambridge, Massachusetts 02139 Previous studies provided evidence that sepsis is associated with increased ubiquitin-proteasome-dependent protein br...

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Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1999-02, Vol.276 (2), p.468
Main Authors: Hobler, Scott C, Wang, Jing Jing, Williams, Arthur B, Melandri, Francesco, Sun, Xiaoyan, Fischer, Josef E, Hasselgren, Per-Olof
Format: Article
Language:English
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Summary:Department of Surgery, University of Cincinnati, Cincinnati, Ohio 45267; Shriners Burns Institute, Cincinnati, Ohio 45229; and ProScript, Incorporated, Cambridge, Massachusetts 02139 Previous studies provided evidence that sepsis is associated with increased ubiquitin-proteasome-dependent protein breakdown in skeletal muscle. The 14-kDa ubiquitin-conjugating enzyme (E2 14k ) has been proposed to be a key regulator of the ubiquitin proteolytic pathway. We tested the hypothesis that E2 14k message and protein levels are increased in skeletal muscle during sepsis. Sepsis was induced in rats by cecal ligation and puncture (CLP). Control rats were sham operated. E2 14k mRNA and protein levels were quantitated after Northern and Western blot analysis, respectively, 16 h after CLP or sham operation. Sepsis resulted in a 70% increase in the 1.2-kb E2 14k transcript in the fast-twitch extensor digitorum longus muscle, whereas no changes were seen in the slow-twitch soleus muscle. E2 14k protein levels were not influenced by sepsis in any of the muscles studied. Although the changes in the expression of the E2 14k 1.2-kb transcript paralleled the differential effect of sepsis on protein breakdown in fast- and slow-twitch muscle, the potential role of E2 14k in the regulation of sepsis-induced muscle proteolysis needs to be interpreted with caution, because the results demonstrated that increased message levels were not associated with increased E2 14k protein levels. proteolysis; cachexia; proteasome
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.1999.276.2.R468