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Estimating glucose metabolism using glucose analogs and two tracer kinetic models in isolated rabbit heart
1 Lawrence Berkeley National Laboratory, University of California, Center for Functional Imaging, Berkeley 94720; 2 Martinez Veterans Affairs, Northern California Health Care System, Martinez 94553; and 3 University of California at Davis, School of Medicine, Davis, California 95616 The purpose o...
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| Published in: | American journal of physiology. Heart and circulatory physiology 1998-08, Vol.275 (2), p.H668-H679 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | 1 Lawrence Berkeley National
Laboratory, University of California, Center for Functional Imaging,
Berkeley 94720; 2 Martinez
Veterans Affairs, Northern California Health Care System, Martinez
94553; and 3 University of
California at Davis, School of Medicine, Davis, California 95616
The purpose of this
investigation was to 1 ) evaluate the
relative accuracy of the Sokoloff and Patlak tracer kinetic models in
estimating glucose metabolic rate (GMR) in the presence and absence of
insulin; 2 ) evaluate the effect of
nutritional state on the lumped constant (LC); and
3 ) compare the kinetics of
2-fluoro-2-deoxy- D -[ 14 C]glucose
(FDG) and
2-deoxy- D -[ 3 H]glucose
(DG) membrane transport and phosphorylation. The experimental preparation was the isolated, red blood cell-albumin-perfused rabbit
heart. Our results showed that both tracer kinetic models provided GMR
estimates that correlated well with the Fick method (for FDG,
R = 0.84 and 0.91 for the Sokoloff and
Patlak models, respectively); nutritional state did not affect the LC;
and FDG and DG have different transport and/or phosphorylation
parameters. We also observed that 1 )
the addition of a fourth compartment to the Sokoloff model reduced the
mean squared error between measured and modeled data by a factor of
7.4; 2 ) a longer time (21.8 min) was
required to obtain a linear phase of the Patlak plot than is allowed in
clinical studies; and 3 ) accurate
GMR estimates were obtained only by using different LCs reflecting
insulin's presence or absence. Our results indicate potential sources
of error in the use of FDG and positron emission tomography to quantify GMR in patients.
fluorodeoxyglucose; deoxyglucose; positron emission tomography |
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| ISSN: | 0363-6135 1522-1539 |
| DOI: | 10.1152/ajpheart.1998.275.2.h668 |