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Thyroid hormone induces activation of mitogen-activated protein kinase in cultured cells
1 Division of Molecular and Cellular Medicine, Department of Medicine, Albany Medical College and 2 Veterans Affairs Healthcare Network Upstate New York, Albany, New York 12208 Thyroid hormone [ L -thyroxine (T 4 )] rapidly induced phosphorylation and nuclear translocation (activation) of mitogen-...
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Published in: | American Journal of Physiology: Cell Physiology 1999-05, Vol.276 (5), p.C1014-C1024 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | 1 Division of Molecular and
Cellular Medicine, Department of Medicine, Albany Medical College and
2 Veterans Affairs Healthcare
Network Upstate New York, Albany, New York 12208
Thyroid hormone
[ L -thyroxine
(T 4 )] rapidly induced
phosphorylation and nuclear translocation (activation) of
mitogen-activated protein kinase (MAPK) in HeLa and CV-1 cells in the
absence of cytokine or growth factor. A pertussis toxin-sensitive and
guanosine 5'- O -(3-thiotriphosphate)-sensitive
cell surface mechanism responsive to
T 4 and
agarose-T 4 , suggesting a G
protein-coupled receptor, was implicated. Cells depleted of MAPK or
treated with MAPK pathway inhibitors showed reduced activation of MAPK
and of the signal transducer and activator of transcription STAT1 by
T 4 ; they also showed
reduced T 4 potentiation of the
antiviral action of interferon- (IFN- ).
T 4 treatment caused
tyrosine-phosphorylated MAPK-STAT1 nuclear complex formation and
enhanced Ser-727 phosphorylation of STAT1 , in the presence or
absence of IFN- . STAT1 -deficient cells transfected with STAT1
containing an alanine-for-serine substitution at residue 727 (STAT1 A727 ) showed minimal
T 4 -stimulated STAT1 activation.
IFN- induced the antiviral state in cells containing wild-type
STAT1 (STAT1 wt ) or
STAT1 A727 ;
T 4 potentiated IFN- action in
STAT1 wt cells but not in
STAT1 A727 cells.
T 4 -directed STAT1 Ser-727
phosphorylation is MAPK mediated and results in potentiated STAT1
activation and enhanced IFN- activity.
thyroxine; signal transducer and activator of transcription 1 ; signal transduction |
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ISSN: | 0363-6143 1522-1563 |
DOI: | 10.1152/ajpcell.1999.276.5.c1014 |