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Histone H2A.z Is Essential for Cardiac Myocyte Hypertrophy but Opposed by Silent Information Regulator 2Î
In this study we have shown that the histone variant H2A.z is up-regulated during cardiac hypertrophy. Upon its knock-down with RNA interference, hypertrophy and the underlying increase in growth-related genes, protein synthesis, and cell size were down-regulated. During attempts to understand the m...
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| Published in: | The Journal of biological chemistry 2006-07, Vol.281 (28), p.19369 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Online Access: | Get full text |
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| Summary: | In this study we have shown that the histone variant H2A.z is up-regulated during cardiac hypertrophy. Upon its knock-down
with RNA interference, hypertrophy and the underlying increase in growth-related genes, protein synthesis, and cell size were
down-regulated. During attempts to understand the mode of regulation of H2A.z, we found that overexpression of silent information
regulator 2alpha (Sir2α) specifically induced down-regulation of H2A.z via NAD-dependent activity. This effect was reversed
by the proteasome inhibitor epoxomicin, suggesting a Sir2α-mediated ubiquitin/proteasome-dependent mechanism for degradation
of H2A.z. An increase in Sir2α also resulted in a dose-dependent reduction of the response to hypertrophic stimuli, whereas
its inhibition resulted in enhanced hypertrophy and apoptosis. We have shown that Sir2α directly deacetylates H2A.z. Mutagenesis
proved that lysines 4, 7, 11, and 13 do not play a role in the stability of H2A.z, whereas Lys-15 was indispensable. Meanwhile,
Lys-115 and conserved, ubiquitinatable Lys-121 are critical for Sir2α-mediated degradation. Fusion of the C terminus of H2A.z
(amino acids 115-127) to H2A.x or green fluorescence protein conferred Sir2α-inducible degradation to the former protein only.
Because H2A.x and H2A.z have conserved N-tails, this implied that both the C and N termini are critical for mediating the
effect of Sir2α. In short, the results suggest that H2A.z is required for cardiac hypertrophy, where its stability and the
extent of cell growth and apoptosis are moderated by Sir2α. We also propose that Sir2α is involved in deacetylation of H2A.z,
which results in ubiquitination of Lys-115 and Lys-121 and its degradation via a ubiquitin/proteasome-dependent pathway. |
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| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M601443200 |