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Presenilin-1 Regulates Intracellular Trafficking and Cell Surface Delivery of β-Amyloid Precursor Protein
Presenilins (PS1/PS2) play a critical role in proteolysis of β-amyloid precursor protein (βAPP) to generate β-amyloid, a peptide important in the pathogenesis of Alzheimer's disease. Nevertheless, several regulatory functions of PS1 have also been reported. Here we demonstrate, in neuroblast...
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Published in: | The Journal of biological chemistry 2003-01, Vol.278 (5), p.3446 |
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Main Authors: | , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | Presenilins (PS1/PS2) play a critical role in proteolysis of β-amyloid precursor protein (βAPP) to generate β-amyloid, a peptide
important in the pathogenesis of Alzheimer's disease. Nevertheless, several regulatory functions of PS1 have also been reported.
Here we demonstrate, in neuroblastoma cells, that PS1 regulates the biogenesis of βAPP-containing vesicles from the trans -Golgi network and the endoplasmic reticulum. PS1 deficiency or the expression of loss-of-function variants leads to robust
vesicle formation, concomitant with increased maturation and/or cell surface accumulation of βAPP. In contrast, release of
vesicles containing βAPP is impaired in familial Alzheimer's disease (FAD)-linked PS1 mutant cells, resulting in reduced βAPP
delivery to the cell surface. Moreover, diminution of surface βAPP is profound at axonal terminals in neurons expressing a
PS1 FAD variant. These results suggest that PS1 regulation of βAPP trafficking may represent an alternative mechanism by which
FAD-linked PS1 variants modulate βAPP processing. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M209065200 |