False-Negative PCR Result Due to Gene Polymorphism: the Example of Neisseria meningitidis

Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctr...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2010-12, Vol.48 (12), p.4590-4591
Main Authors: Jaton, Katia, Ninet, Béatrice, Bille, Jacques, Greub, Gilbert
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Child, Preschool
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
False Negative Reactions
Fundamental and applied biological sciences. Psychology
Humans
Male
Meningococcal Infections - diagnosis
Meningococcal Infections - microbiology
Microbiology
Miscellaneous
Molecular Sequence Data
Neisseria meningitidis
Neisseria meningitidis - genetics
Neisseria meningitidis - isolation & purification
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Sequence Analysis, DNA
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Summary:Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctrA gene.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01766-10