High-Level Biosynthesis of the Anteiso-C₁₇ Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity

High-Level Biosynthesis of the Anteiso-C₁₇ Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity

High-level production (880 mg liter⁻¹) and isolation of the anteiso-C₁₇ isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggest...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2009-07, Vol.75 (13), p.4636-4640
Main Authors: Fickers, Patrick, Guez, Jean-Sébastien, Damblon, Christian, Leclère, Valérie, Béchet, Max, Jacques, Philippe, Joris, Bernard
Format: Article
Language:eng
Subjects:
Antibiotics
Bacillus subtilis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Life Sciences
Medication
Microbiology
Microbiology and Parasitology
Pharmaceutical sciences
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Summary:High-level production (880 mg liter⁻¹) and isolation of the anteiso-C₁₇ isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.
ISSN:0099-2240
1098-5336
1098-6596