Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3,1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking i...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1997-08, Vol.63 (8), p.3336-3340
Main Authors: Heng, N.C.K, Jenkinson, H.F, Tannock, G.W
Format: Article
Language:English
Subjects:
Bacillus - genetics
Bacillus macerans
beta-Galactosidase - metabolism
Biological and medical sciences
Biotechnology
Cloning
Cloning, Molecular
DNA Probes - genetics
Endo-1,3-beta-Glucanase
feed processing
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes
Genetic engineering
Genetic technics
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Lactobacillus - genetics
Lactobacillus - metabolism
Lactobacillus reuteri
Methods. Procedures. Technologies
Microbiology
Modification of gene expression level
Plasmids
Promoter Regions, Genetic - genetics
Recombination, Genetic
Restriction Mapping
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Summary:Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3,1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.63.8.3336-3340.1997