Factors limiting aliphatic chlorocarbon degradation by Nitrosomonas europaea: cometabolic inactivation of ammonia monooxygenase and substrate specificity

The son nitrifying bacterium Nitrosomonas europaea is capable of degrading trichloroethylene. (TCE) and other halogenated hydrocarbons. TCE cometabolism by N. europaea resulted in an irreversible loss of TCE biodegradative capacity, ammonia-oxidizing activity, and ammonia-dependent O2, uptake by the...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1991-10, Vol.57 (10), p.2986-2994
Main Authors: RASCHE, M. E, HYMAN, M. R, ARP, D. J
Format: Article
Language:English
Subjects:
560300 > Chemicals Metabolism & Toxicology
actividad enzimatica
activite enzymatique
AMMONIA
ammonia monooxygenase
ammoniac
amoniaco
Analytical, structural and metabolic biochemistry
BACTERIA
biodegradacion
BIODEGRADATION
Biological and medical sciences
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
ENVIRONMENTAL SCIENCES
enzimas
enzyme
ENZYME INHIBITORS
ENZYMES
Enzymes and enzyme inhibitors
enzymic activity
flora del suelo
flore du sol
Fundamental and applied biological sciences. Psychology
HALOGENATED ALIPHATIC HYDROCARBONS
halogenated hydrocarbons
hidrocarburos halogenados
HYDRIDES
hydrocarbure halogene
HYDROGEN COMPOUNDS
INACTIVATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
Physiology and Biotechnology
PROTEINS 540220 > Environment, Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
radioactive labelling
soil flora
SUBSTRATES
TOXICITY
TRACER TECHNIQUES
trichloroethylene
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Summary:The son nitrifying bacterium Nitrosomonas europaea is capable of degrading trichloroethylene. (TCE) and other halogenated hydrocarbons. TCE cometabolism by N. europaea resulted in an irreversible loss of TCE biodegradative capacity, ammonia-oxidizing activity, and ammonia-dependent O2, uptake by the cells. inactivation was not observed in the presence of allylthiourea, a specific inhibitor of the enzyme ammonia monooxygenase, or under anaerobic conditions, indicating that the TCE-mediated inactivation required ammonia monooxygenase activity. When N. europaea cells were incubated with [14C]TCE under conditions which allowed turnover of ammonia monooxygenase, a number of cellular proteins were covalently labeled with 14C. Treatment of cells with allylthiourea or acetylene prior to incubation with [14C]TCE prevented incorporation of 14C into proteins. The ammonia-oxidizing activity of cells inactivated in the presence of TCE could be recovered through a process requiring de novo protein synthesis. In addition to TCE, a series of chlorinated methanes, ethanes, and other ethylenes were screened as substrates for ammonia monooxygenase and for their ability to inactivate the ammonia-oxidizing system of N. europaea. The chlorocarbons could be divided into three classes depending on their biodegradability and inactivating potential: (i) compounds which were not biodegradable by N. europaea and which had no toxic effect on the cells; (ii) compounds which were cooxidized by N. europaea and had little or no toxic effect on the cells; and (iii) compounds which were cooxidized and produced a turnover-dependent inactivation of ammonia oxidation by N. europaea.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.57.10.2986-2994.1991