A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier

Listeria monocytogenes is responsible for severe food-borne infections, but the mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria monocytogenes expresses a surface protein, internalin, that interacts with a host receptor, E-cadherin, to promote entry into human epitheli...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2001-06, Vol.292 (5522), p.1722-1725
Main Authors: Lecuit, Marc, Vandormael-Pournin, Sandrine, Lefort, Jean, Huerre, Michel, Gounon, Pierre, Dupuy, Catherine, Babinet, Charles, Cossart, Pascale
Format: Article
Language:English
Subjects:
Analysis
Anatomy
Animals
B lymphocytes
Bacteria
Bacterial diseases
Bacterial infections
Bacterial load
Bacterial Proteins
Bacterial Proteins - metabolism
Bacterial Translocation
Bacteriology
Biological and medical sciences
Cadherins
Cadherins - genetics
Cadherins - metabolism
Carrier Proteins
Carrier Proteins - genetics
Cells
Colony Count, Microbial
Disease Models, Animal
Enterocytes
Enterocytes - metabolism
Enterocytes - microbiology
Epithelial cells
Experimental bacterial diseases and models
Fatty Acid-Binding Protein 7
Fatty Acid-Binding Proteins
Foodborne diseases
Guinea Pigs
Human health and pathology
Humans
Infections
Infectious diseases
internalin
Intestinal Mucosa
Intestinal Mucosa - microbiology
Intestinal Mucosa - pathology
Intestine, Small
Intestine, Small - microbiology
Intestine, Small - pathology
Life Sciences
Listeria monocytogenes
Listeria monocytogenes - growth & development
Listeria monocytogenes - metabolism
Listeria monocytogenes - pathogenicity
Listeriosis
Listeriosis - microbiology
Listeriosis - pathology
Liver
Liver - microbiology
Liver - pathology
Lymph Nodes
Lymph Nodes - microbiology
Lymph Nodes - pathology
Male
Medical sciences
Mice
Mice, Transgenic
Microbiology and Parasitology
Neoplasm Proteins
Nerve Tissue Proteins
Promoter Regions, Genetic
Proteins
Receptors
Rodents
Spleen
Spleen - microbiology
Spleen - pathology
Transgenes
Transgenic animals
Tumor Suppressor Proteins
Virulence
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Description
Summary:Listeria monocytogenes is responsible for severe food-borne infections, but the mechanisms by which bacteria cross the intestinal barrier are unknown. Listeria monocytogenes expresses a surface protein, internalin, that interacts with a host receptor, E-cadherin, to promote entry into human epithelial cells. Murine E-cadherin, in contrast to guinea pig E-cadherin, does not interact with internalin, excluding the mouse as a model for addressing internalin function in vivo. In guinea pigs and transgenic mice expressing human E-cadherin, internalin was found to mediate invasion of enterocytes and crossing of the intestinal barrier. These results illustrate how relevant animal models for human infections can be generated.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1059852