Continuous enzymatic solubilization of alfalfa proteins in an ultrafiltration reactor

Insoluble alfalfa protein concentrate (APC) was solubilised by two proteolytic enzymes (pepsin and Delvolase) in batch and in continuous flow membrane reactor. In a preliminary investigation, the suitable enzyme (Delvolase) was selected and the nitrogen solubility profile of APC was established. The...

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Bibliographic Details
Published in:Enzyme and microbial technology 2004-04, Vol.34 (5), p.380-391
Main Authors: Prevot-D’Alvise, N., Lesueur-Lambert, C., Fertin-Bazus, A., Fertin, B., Dhulster, P., Guillochon, D.
Format: Article
Language:English
Subjects:
Alfalfa protein concentrate
Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts
Biological and medical sciences
Bioreactors
Biotechnology
Chemical and Process Engineering
Delvolase
Engineering Sciences
Food industries
Fundamental and applied biological sciences. Psychology
Hydrolyse
Methods. Procedures. Technologies
Peptides
Ultrafiltration
Various methods and equipments
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Summary:Insoluble alfalfa protein concentrate (APC) was solubilised by two proteolytic enzymes (pepsin and Delvolase) in batch and in continuous flow membrane reactor. In a preliminary investigation, the suitable enzyme (Delvolase) was selected and the nitrogen solubility profile of APC was established. The kinetics of APC solubilization was investigated: no product inhibition was noticed and the enzyme was found to be adsorbed on the APC particles. The effect of operating variables such as enzyme and substrate concentrations, permeate flow, reactor volume and residence time were studied.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2003.05.001