Pyruvate modifies glycolytic and oxidative metabolism of rat embryonic spinal cord astrocyte cell lines and prevents their spontaneous transformation

This study aimed to provide detailed data on mitochondrial respiration of normal astrocyte cell lines derived from rat embryonic spinal cord. Astrocytes in early passages (EP), cultured without pyruvate for more than 35 passages, defined here as late passages (LP), undergo spontaneous transformation...

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Bibliographic Details
Published in:Journal of neurochemistry 2007-03, Vol.100 (6), p.1589-1598
Main Authors: Rouleau, Caroline, Rakotoarivelo, Clovis, Petite, Didier, Lambert, Karen, Fabre, Christine, Bonardet, Andrée, Mercier, Jacques, Baldet, Pierre, Privat, Alain, Langley, Keith, Mersel, Marcel
Format: Article
Language:English
Subjects:
Aging
Aging - drug effects
Aging - physiology
Animals
Astrocytes
Astrocytes - drug effects
Astrocytes - ultrastructure
Biochemistry
Biological and medical sciences
Cells, Cultured
Cellular biology
Electron Transport Chain Complex Proteins
Electron Transport Chain Complex Proteins - metabolism
Embryo
Embryo, Mammalian
Fundamental and applied biological sciences. Psychology
Glycolysis
Glycolysis - drug effects
Injuries of the nervous system and the skull. Diseases due to physical agents
Isolated neuron and nerve. Neuroglia
Life Sciences
Medical sciences
Mitochondria
Mitochondria - drug effects
Neurons and Cognition
Neurosciences
Oxygen Consumption
Oxygen Consumption - drug effects
pyruvate
Pyruvic Acid
Pyruvic Acid - metabolism
Pyruvic Acid - pharmacology
Rats
Spinal Cord
Spinal Cord - cytology
spontaneous transformation
Traumas. Diseases due to physical agents
Vertebrates: nervous system and sense organs
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Summary:This study aimed to provide detailed data on mitochondrial respiration of normal astrocyte cell lines derived from rat embryonic spinal cord. Astrocytes in early passages (EP), cultured without pyruvate for more than 35 passages, defined here as late passages (LP), undergo spontaneous transformation. To study initial steps in cell transformation, EP data were compared with those of LP cells. LP cells had reduced glycolysis, fewer mitochondria and extremely low oxidative rates, resulting from a dysfunction of complexes I and II + III of the respiratory chain. Treatment of EP cells with pyruvate until they were, by definition, LP cultures prevented transformation of these cells. Pyruvate‐treated EP cells had more mitochondria than normal cells but slightly lower respiratory rates. The increase of mitochondrial content thus appears to act as a compensatory effect to maintain oxidative phosphorylation in these LP ‘non‐transformed’ cells, in which mitochondrial function is reduced. However, pyruvate treatment of transformed LP cells during additional passages did not significantly restore their oxidative metabolism. These data highlight changes accompanying spontaneous astrocyte transformation and suggest potential targets for the control of astrocyte proliferation and reaction to various insults to the central nervous system.
ISSN:0022-3042
1471-4159
DOI:10.1111/j.1471-4159.2006.04318.x