Contribution of the Plasmin/Matrix Metalloproteinase Cascade to the Retraction of Human Fibroblast Populated Collagen Lattices

To assess the contribution of the plasmin/matrix metalloproteinase cascade in lattices retraction, human gingival fibroblast-populated collagen lattices were supplemented with plasminogen. The rate of lattice retraction was enhanced by addition of plasminogen. This effect was concomitant to plasmin...

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Published in:Molecular cell biology research communications 2000-03, Vol.3 (3), p.173-180
Main Authors: Berton, A., Lorimier, S., Emonard, H., Laurent-Maquin, D., Hornebeck, W., Bellon, G.
Format: Article
Language:English
Subjects:
Bioengineering
Cells, Cultured
Collagen
Collagen - metabolism
Enzyme Activation
Fibrinolysin - metabolism
Fibroblasts
Fibroblasts - enzymology
Fibroblasts - metabolism
Fibroblasts - ultrastructure
Gingiva
Gingiva - enzymology
Gingiva - metabolism
Gingiva - ultrastructure
Humans
Life Sciences
Matrix Metalloproteinases
Matrix Metalloproteinases - metabolism
Microscopy, Electron, Scanning
Plasmin
Research Support, Non-U.S. Gov't
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Summary:To assess the contribution of the plasmin/matrix metalloproteinase cascade in lattices retraction, human gingival fibroblast-populated collagen lattices were supplemented with plasminogen. The rate of lattice retraction was enhanced by addition of plasminogen. This effect was concomitant to plasmin generation, prostromelysin-1 and procollagenase activation. Plasminogen-mediated initiation of that proteolytic cascade was accompanied by conspicuous changes in cell morphology and collagen fibers organization. At day 1 of culture fibroblasts shifted from a rounded (control) to an elongated (in presence of plgn) shape. At the latest stage of retraction, intense vacuolization around fibroblasts was noticed in plgn-supplemented lattices which paralleled the increased collagen degradation. Plgn-enhancing influence on the initial phase of lattice retraction could be totally annihilated by either aprotinin or Batimastat. Those data emphasize the crucial importance of the plasmin–MMP proteolytic cascade in granulation tissue retraction in a healing wound.
ISSN:1522-4724
1522-4732
DOI:10.1006/mcbr.2000.0210