An integrative approach combining passive sampling, bioassays, and effect‐directed analysis to assess the impact of wastewater effluent

Wastewater treatment plant (WWTP) effluents are major sources of endocrine‐disrupting chemicals (EDCs) and other chemicals of toxicological concern for the aquatic environment. In the present study, we used an integrated strategy combining passive sampling (Chemcatcher®), developmental toxicity, and...

Full description

Saved in:
Bibliographic Details
Published in:Environmental toxicology and chemistry 2018-08, Vol.37 (8), p.2079-2088
Main Authors: Sonavane, Manoj, Schollée, Jennifer E., Hidasi, Anita O., Creusot, Nicolas, Brion, François, Suter, Marc J.‐F., Hollender, Juliane, Aїt‐Aїssa, Selim
Format: Article
Language:English
Subjects:
17β-Estradiol
Aquatic environment
Aromatase
Aromatic compounds
Bioassays
Biocompatibility
Chemicals
Danio rerio
Ecotoxicology
Effect‐directed analysis
Effluents
Embryo toxicity
Endocrine active compounds
Endocrine disruptors
Endocrine‐disrupting chemicals (EDCs)
Estrogens
Estrone
Ethinylestradiol
Fluorescence
Fluticasone
Gene expression
Glucocorticoids
Green fluorescent protein
Hazard identification
In vivo methods and tests
Integrated approach
LC‐MS/MS analysis
Life Sciences
Organic chemistry
Passive sampling
Propionic acid
Proteins
Reporter gene
Rivers
Sampling
Sex hormones
Teratogenicity
Toxic hazards
Toxicity
Toxicity testing
Toxicology
Wastewater treatment
Wastewater treatment plants
Water treatment plants
WWTP effluent
Xenoestrogens
Zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Wastewater treatment plant (WWTP) effluents are major sources of endocrine‐disrupting chemicals (EDCs) and other chemicals of toxicological concern for the aquatic environment. In the present study, we used an integrated strategy combining passive sampling (Chemcatcher®), developmental toxicity, and mechanism‐based in vitro and in vivo bioassays to monitor the impacts of a WWTP on a river. In vitro screening revealed the WWTP effluent as a source of estrogen, glucocorticoid, and aryl hydrocarbon (AhR) receptor‐mediated activities impacting the downstream river site where significant activities were also measured, albeit to a lesser extent than in the effluent. Effect‐directed analysis of the effluent successfully identified the presence of potent estrogens (estrone, 17α‐ethinylestradiol, and 17β‐estradiol) and glucocorticoids (clobetasol propionate and fluticasone propionate) as the major contributors to the observed in vitro activities, even though other unidentified active chemicals were likely present. The impact of the WWTP was also assessed using zebrafish embryo assays, highlighting its ability to induce estrogenic response through up‐regulation of the aromatase promoter‐dependent reporter gene in the transgenic (cyp19a1b–green fluorescent protein [GFP]) zebrafish assay and to generate teratogenic effects at nonlethal concentrations in the zebrafish embryo toxicity test. The present study argues for the use of such an integrated approach, combining passive sampling, bioassays, and effect‐directed analysis, to comprehensively identify endocrine active compounds and associated hazards of WTTP effluents. Environ Toxicol Chem 2018;37:2079–2088. © 2018 SETAC
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.4155