The Krüppel-like core promoter binding protein gene is primarily expressed in placenta during mouse development

The human core promoter binding protein (hCPBP) has been identified as a DNA-binding protein involved in the regulation of TATA box-less genes like those encoding the pregnancy-specific glycoproteins. Structurally, hCPBP contains three zinc fingers in the C-terminal domain, which is highly conserved...

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Bibliographic Details
Published in:Biology of reproduction 1999-12, Vol.61 (6), p.1586-1591
Main Authors: SLAVIN, D, SAPIN, V, LOPEZ-DIAZ, F, JACQUEMIN, P, KORITSCHONER, N, DASTUGUE, B, DAVIDSON, I, CHATTON, B, BOCCO, J. L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Cloning, Molecular
Conserved Sequence
Embryology: invertebrates and vertebrates. Teratology
Embryonic and Fetal Development
Female
Fetal membranes
Fundamental and applied biological sciences. Psychology
Gene Expression
General aspects. Development. Fetal membranes
Humans
In Situ Hybridization
Kruppel-Like Factor 6
Kruppel-Like Transcription Factors
Life Sciences
Mice
Molecular Sequence Data
Placenta - chemistry
Placenta - metabolism
Pregnancy
Proto-Oncogene Proteins
Reproductive Biology
RNA, Messenger - analysis
Trans-Activators - chemistry
Trans-Activators - genetics
Zinc Fingers
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Summary:The human core promoter binding protein (hCPBP) has been identified as a DNA-binding protein involved in the regulation of TATA box-less genes like those encoding the pregnancy-specific glycoproteins. Structurally, hCPBP contains three zinc fingers in the C-terminal domain, which is highly conserved in a number of proteins that constitute the Krüppel-like family of transcription factors. In the present work, we report the molecular cloning of the mouse CPBP (mCPBP) and its expression pattern during development as well as in adult tissues. The mouse cDNA encodes a protein of 283 amino acids that share 94.4% of identity with the hCPBP. The highest level of mCPBP transcript was detected in placenta, and its expression was lower in total embryos and in adult tissues. We also show by in situ hybridization that during embryonic development the mCPBP gene is mainly expressed in extra-embryonic structures throughout gestation; essentially no specific expression was detected in embryonic tissues. Our data demonstrate that CPBP transcript is enriched in the trophoblastic tissue and strongly suggest that its encoded polypeptide regulates target genes involved in placental development and pregnancy maintenance.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod61.6.1586