Co-inherited novel SNPs of the LIPE gene associated with increased carcass dressing and decreased fat-tail weight in Awassi breed

The lipase E hormone-sensitive (LIPE) enzyme is one of the lipolytic enzymes, and it plays a key role in the regulation of adipose tissue deposition. This study was conducted to investigate the possible association between the LIPE gene variations and the main body weight measurements in Awassi shee...

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Bibliographic Details
Published in:Tropical animal health and production 2020-11, Vol.52 (6), p.3631-3638
Main Authors: Al-Thuwaini, Tahreer M., Al-Shuhaib, Mohammed Baqur S., Lepretre, Frederic, Mahdi, Zainab A.
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - metabolism
Animals
Association analysis
Biomedical and Life Sciences
Body weight
Carcasses
Deoxyribonucleic acid
DNA
Gene polymorphism
Genotyping
Haplotypes
Heredity
Iraq
Life Sciences
Linkage analysis
Linkage Disequilibrium
Lipase
Lipoprotein Lipase - genetics
Lipoprotein Lipase - metabolism
Male
Meat - analysis
Metabolism
Nucleotides
Ovis aries
Polymerase chain reaction
Polymorphism
Polymorphism, Single Nucleotide
Regular Articles
Sheep
Sheep, Domestic - metabolism
Sheep, Domestic - physiology
Single-nucleotide polymorphism
Veterinary Medicine/Veterinary Science
Zoology
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Summary:The lipase E hormone-sensitive (LIPE) enzyme is one of the lipolytic enzymes, and it plays a key role in the regulation of adipose tissue deposition. This study was conducted to investigate the possible association between the LIPE gene variations and the main body weight measurements in Awassi sheep. A total of 160 of sexually mature Awassi rams ( Ovis aries ) that aged between 2 and 3 years were included in the present study. Genomic DNA was extracted and two specific PCR amplicons were designed to amplify two coding regions within the LIPE gene. Genotyping experiments were performed using polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP). Two different SSCP banding patterns were identified, CC and CD in exon 2, and AA and AT in exon 9. Five novel single-nucleotide polymorphisms (SNPs) were detected by sequencing, namely g.151C > A and g.198C > T in exon 2, and g.213G > C, g.226G > T, and g.232A > C in exon 9. Haplotype block analysis showed strong linkage disequilibrium values between the two SNPs in exon 2 and the three SNPs in exon 9. Association analysis of haplotypes with carcass traits demonstrated a significantly higher dressing percentage ( P  
ISSN:0049-4747
1573-7438
1573-7438
DOI:10.1007/s11250-020-02400-9