Susceptibility of adult Phlyctinus (Coleoptera: Curculionidae) to entomopathogens: A first look at potential differences in a newly revised species complex

Phlyctinus callosus and P. xerophilus (Coleoptera: Curculionidae) are two cryptic species of native entimine weevils, previously grouped together under the P. callosus sensu lato concept, that are pests of economic importance to the deciduous fruit and vine industry in the Western Cape province of S...

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Bibliographic Details
Published in:Journal of applied entomology (1986) 2024-03, Vol.148 (2), p.129-139
Main Authors: Hansen, Steffan P., Malan, Antoinette P., Haran, Julien M., Addison, Pia
Format: Article
Language:English
Subjects:
Bioassays
Biodiversity
Biological control
Cadavers
Coleoptera
Conidia
Cryptic species
Curculionidae
Economic importance
Entomopathogenic fungi
Entomopathogenic nematodes
EPF
EPN
Filter paper
Genetics
Indigenous species
Inoculation
Insects
integrated pest management
Life Sciences
Mycosis
Pathogenicity
Pathogens
Peltodytes callosus
Pests
Phlyctinus callosus
Phlyctinus xerophilus
Populations and Evolution
Susceptibility
Systematics, Phylogenetics and taxonomy
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Summary:Phlyctinus callosus and P. xerophilus (Coleoptera: Curculionidae) are two cryptic species of native entimine weevils, previously grouped together under the P. callosus sensu lato concept, that are pests of economic importance to the deciduous fruit and vine industry in the Western Cape province of South Africa. Laboratory bioassays were conducted using entomopathogenic fungi (EPF) isolates of Beauveria and entomopathogenic nematodes (EPNs), Heterorhabditis indica and Steinernema yirgalemense, to determine differences in susceptibility of adult P. callosus and P. xerophilus to potential biological control agents. The test arena used was 24‐well bioassay plates with an inoculation rate of 200 infective juveniles (IJs)/insect for EPNs and 5 × 105 conidia/insect for EPF. Insects were inoculated using a 12.7 mm filter paper impregnated with 50 μL of entomopathogen suspension. Infection was determined after 96 h incubation for EPNs through dissection of cadavers. Insects inoculated with EPF were incubated in the wells for 18 days and mortality recorded daily. Cadavers were surface sterilized and observed for overt mycosis. Differential susceptibility between P. callosus and P. xerophilus was observed in EPF but not in EPN bioassays. Differential susceptibility to EPF could be due to methodology. Low adult weevil pathogenicity was found for all entomopathogens, with variable results obtained from different bioassay batches.
ISSN:0931-2048
1439-0418
DOI:10.1111/jen.13214