Validation of antibody-based recognition by piezoelectric transducers through electroacoustic admittance analysis

The development of immunosensors based on piezoelectric transducers is widely investigated due to their attractive potentialities. The quartz crystal microbalance (QCM) may give a direct response signal which characterizes the binding event between a sensitive layer, immobilized onto the surface tra...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1998-03, Vol.13 (3), p.259-269
Main Authors: Bizet, K., Gabrielli, C., Perrot, H., Therasse, J.
Format: Article
Language:English
Subjects:
Acoustic Impedance Tests
Adsorption
Antigen-Antibody Reactions
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Chemical Sciences
electroacoustic admittance
Electrodes
Electronics, Medical
enzymes adsorption
Fundamental and applied biological sciences. Psychology
Gold
immunosensors
Methods. Procedures. Technologies
Peroxidase - analysis
Proteins - chemistry
QCM
Quartz
Reproducibility of Results
Transducers
Various methods and equipments
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Summary:The development of immunosensors based on piezoelectric transducers is widely investigated due to their attractive potentialities. The quartz crystal microbalance (QCM) may give a direct response signal which characterizes the binding event between a sensitive layer, immobilized onto the surface transducer, and the analyte to be detected. However, for small biomolecules, such as some antigens, it is quite difficult to obtain an observable signal. This is mainly due to the lack of sensitivity of the commonly used QCM (5 to 10 MHz quartz crystal). Moreover, the mass estimated with the QCM response through the Sauerbrey equation and the mass which can be measured thanks to other analytical techniques, in our case an enzymatic assay, are different: the deposited mass is generally overestimated by the QCM. To validate QCM mass measurements and, therefore antigens recognition, the interactions of acoustic shear waves with a biolayer were investigated during enzyme adsorption onto the microbalance gold electrode or during the antibody/antigen binding. Electroacoustic admittance was measured around the resonance frequency of a 27 MHz quartz resonator in parallel with microbalance measurements. The parameters which characterize the quartz microbalance equivalent circuit were compared with the classical microbalance frequency. The mass overestimation, given by the microbalance, could be explained either by modification of the rheological properties of the sensitive layers and/or by an inadequacy of the assay performed.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(97)00139-5