Effect of subtherapeutic and therapeutic sulfamethazine concentrations on transcribed genes and translated proteins involved in Microbacterium sp. C448 resistance and degradation

Abstract Microbacterium sp. C448, isolated from a soil regularly exposed to sulfamethazine (SMZ), can use various sulphonamide antibiotics as the sole carbon source for growth. The basis for the regulation of genes encoding the sulphonamide metabolism pathway, the dihydropteroate synthase sulphonami...

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Bibliographic Details
Published in:FEMS microbiology ecology 2023-06, Vol.99 (7)
Main Authors: Paris, Laurianne, Devers-Lamrani, Marion, Joly, Muriel, Viala, Didier, De Antonio, Marie, Pereira, Bruno, Rouard, Nadine, Besse-Hoggan, Pascale, Hébraud, Michel, Topp, Edward, Martin-Laurent, Fabrice, Batisson, Isabelle
Format: Article
Language:English
Subjects:
Aminophenol
Anti-Infective Agents - metabolism
Antibiotics
Bacteria
Biodegradation
Biodegradation, Environmental
Carbon sources
Degradation
Detoxification
Dihydropteroate synthase
Dihydropteroate Synthase - genetics
Dihydropteroate Synthase - metabolism
Drug Resistance, Bacterial
Exposure
Gene regulation
Genes
Gram-positive bacteria
Kinetics
Life Sciences
Metabolites
Microbacterium
Microbacterium - genetics
Microbacterium - metabolism
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
p-Aminophenol
Protein folding
Proteins
Proteome
Proteomes
Proteomics
R&D
Research & development
Soil Microbiology
Sulfamethazine
Sulfamethazine - metabolism
Sulfates
Sulfonamides
Sulfonamides - metabolism
Transcriptome
Transcriptomes
Transcriptomics
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Summary:Abstract Microbacterium sp. C448, isolated from a soil regularly exposed to sulfamethazine (SMZ), can use various sulphonamide antibiotics as the sole carbon source for growth. The basis for the regulation of genes encoding the sulphonamide metabolism pathway, the dihydropteroate synthase sulphonamide target (folP), and the sulphonamide resistance (sul1) genes is unknown in this organism. In the present study, the response of the transcriptome and proteome of Microbacterium sp. C448 following exposure to subtherapeutic (33 µM) or therapeutic (832 µM) SMZ concentrations was evaluated. Therapeutic concentration induced the highest sad expression and Sad production, consistent with the activity of SMZ degradation observed in cellulo. Following complete SMZ degradation, Sad production tended to return to the basal level observed prior to SMZ exposure. Transcriptomic and proteomic kinetics were concomitant for the resistance genes and proteins. The abundance of Sul1 protein, 100-fold more abundant than FolP protein, did not change in response to SMZ exposure. Moreover, non-targeted analyses highlighted the increase of a deaminase RidA and a putative sulphate exporter expression and production. These two novel factors involved in the 4-aminophenol metabolite degradation and the export of sulphate residues formed during SMZ degradation, respectively, provided new insights into the Microbacterium sp. C448 SMZ detoxification process. Transcriptomic and proteomic responses of Microbacterium sp. C448 exposed to sulfamethazine antibiotic.
ISSN:0168-6496
1574-6941
DOI:10.1093/femsec/fiad064