M101, a therapeutic oxygen carrier derived from Arenicola marina, decreased Porphyromonas gingivalis‐induced hypoxia and improved periodontal healing

Background Porphyromonas gingivalis exacerbates tissue hypoxia and worsens periodontal inflammation. This study investigated the effect of a therapeutic oxygen carrier (M101), derived from Arenicola marina, on hypoxia and associated inflammation in the context of periodontitis. Methods The effect of...

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Published in:Journal of periodontology (1970) 2022-11, Vol.93 (11), p.1712-1724
Main Authors: Batool, Fareeha, Petit, Catherine, Stutz, Céline, Özçelik, Hayriye, Gegout, Pierre‐Yves, Benkirane‐Jessel, Nadia, Delpy, Eric, Zal, Franck, Leize‐Zal, Elisabeth, Huck, Olivier
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Antioxidants - pharmacology
Antioxidants - therapeutic use
Arenicola marina
Bioengineering
hemoglobin
Hypoxia - metabolism
Inflammation
Life Sciences
Matrix Metalloproteinase 9 - metabolism
Mice
NF-kappa B - metabolism
oxidative stress
Oxygen - metabolism
Oxygen - therapeutic use
periodontitis
Periodontitis - drug therapy
Periodontitis - metabolism
Porphyromonas gingivalis - metabolism
Wound Healing
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Summary:Background Porphyromonas gingivalis exacerbates tissue hypoxia and worsens periodontal inflammation. This study investigated the effect of a therapeutic oxygen carrier (M101), derived from Arenicola marina, on hypoxia and associated inflammation in the context of periodontitis. Methods The effect of M101 on GLUT‐1, GLUT‐3, HIF‐1α, and MMP‐9 expression, hypoxia, and antioxidant status in oral epithelial cells (EC) exposed to CoCl2 (1000 μM), P. gingivalis (MOI 100), and CoCl2 + P. gingivalis was evaluated through hypoxia detection fluorescence assay, antioxidant concentration colorimetric assay, and RTqPCR. Evaluation of M101 on EC proliferation was evaluated in an in vitro wound assay. In experimental periodontitis, periodontal wound healing and osteoclastic activity were compared among natural wound healing, placebo, and gels containing M101 (1  and 2 g/L) groups through histomorphometry and TRAP (tartrate‐resistant acid phosphatase activity assay) assay respectively. The expression of HIF‐1α, MMP‐9, and NFκB in periodontal tissues was also evaluated through immunofluorescence studies. Results M101 downregulated GLUT‐1, GLUT‐3, HIF‐1α, and MMP‐9 levels in EC exposed to CoCl2, P. gingivalis, and CoCl2 + P. gingivalis (p 
ISSN:0022-3492
1943-3670
DOI:10.1002/JPER.22-0006