Usefulness of matrix-assisted laser desorption ionisation–time-of-flight mass spectrometry for identifying clinical Trichosporon isolates

Trichosporon spp. have recently emerged as significant human pathogens. Identification of these species is important, both for epidemiological purposes and for therapeutic management, but conventional identification based on biochemical traits is hindered by the lack of updates to the species databa...

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Bibliographic Details
Published in:Clinical microbiology and infection 2014-08, Vol.20 (8), p.784-790
Main Authors: de Almeida Júnior, J.N., Figueiredo, D.S.Y., Toubas, D., Del Negro, G.M.B., Motta, A.L., Rossi, F., Guitard, J., Morio, F., Bailly, E., Angoulvant, A., Mazier, D., Benard, G., Hennequin, C.
Format: Article
Language:English
Subjects:
Fungal Proteins - chemistry
Fungal Proteins - isolation & purification
Humans
Identification
Life Sciences
main spectrum database
MALDI–TOF mass spectrometry
protein extraction procedures
Sensitivity and Specificity
Specimen Handling - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Trichosporon
Trichosporon - chemistry
Trichosporon - classification
Trichosporon - isolation & purification
Trichosporonosis - diagnosis
Trichosporonosis - microbiology
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Summary:Trichosporon spp. have recently emerged as significant human pathogens. Identification of these species is important, both for epidemiological purposes and for therapeutic management, but conventional identification based on biochemical traits is hindered by the lack of updates to the species databases provided by the different commercial systems. In this study, 93 strains, or isolates, belonging to 16 Trichosporon species were subjected to both molecular identification using IGS1 gene sequencing and matrix-assisted laser desorption ionisation–time-of-flight (MALDI–TOF) analysis. Our results confirmed the limits of biochemical systems for identifying Trichosporon species, because only 27 (36%) of the isolates were correctly identified using them. Different protein extraction procedures were evaluated, revealing that incubation for 30 min with 70% formic acid yields the spectra with the highest scores. Among the six different reference spectra databases that were tested, a specific one composed of 18 reference strains plus seven clinical isolates allowed the correct identification of 67 of the 68 clinical isolates (98.5%). Although until recently it has been less widely applied to the basidiomycetous fungi, MALDI–TOF appears to be a valuable tool for identifying clinical Trichosporon isolates at the species level.
ISSN:1198-743X
1469-0691
DOI:10.1111/1469-0691.12502