Molecular Imaging of Atherosclerotic Plaques Using a Human Antibody Against the Extra-Domain B of Fibronectin

Current imaging modalities of human atherosclerosis, such as angiography, ultrasound, and computed tomography, visualize plaque morphology. However, methods that provide insight into plaque biology using molecular tools are still insufficient. The extra-domain B (ED-B) is inserted into the fibronect...

Full description

Saved in:
Bibliographic Details
Published in:Circulation research 2004-12, Vol.95 (12), p.1225-1233
Main Authors: Matter, Christian M, Schuler, Pia K, Alessi, Patrizia, Meier, Patricia, Ricci, Romeo, Zhang, Dongming, Halin, Cornelia, Castellani, Patrizia, Zardi, Luciano, Hofer, Christoph K, Montani, Matteo, Neri, Dario, Lüscher, Thomas F
Format: Article
Language:English
Subjects:
Adult
Animals
Antibodies, Monoclonal - immunology
Antibody Affinity
Aorta - chemistry
Aorta - ultrastructure
Aorta, Abdominal - chemistry
Aorta, Abdominal - ultrastructure
Aortic Diseases - metabolism
Aortic Diseases - pathology
Apolipoproteins E - deficiency
Apolipoproteins E - genetics
Arteriosclerosis - metabolism
Arteriosclerosis - pathology
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Carbocyanines
Cardiology. Vascular system
Coronary Vessels - chemistry
Coronary Vessels - ultrastructure
Diet, Atherogenic
Fibronectins - analysis
Fibronectins - immunology
Fluorescent Dyes - analysis
Fundamental and applied biological sciences. Psychology
Humans
Iliac Artery - chemistry
Iliac Artery - ultrastructure
Life Sciences
Male
Mammary Arteries - chemistry
Mammary Arteries - ultrastructure
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Fluorescence
Middle Aged
Protein Structure, Tertiary
Radioimmunodetection
Recombinant Proteins - immunology
Spectroscopy, Near-Infrared
Vasa Vasorum - chemistry
Vasa Vasorum - ultrastructure
Vertebrates: cardiovascular system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Current imaging modalities of human atherosclerosis, such as angiography, ultrasound, and computed tomography, visualize plaque morphology. However, methods that provide insight into plaque biology using molecular tools are still insufficient. The extra-domain B (ED-B) is inserted into the fibronectin molecule by alternative splicing during angiogenesis and tissue remodeling but is virtually undetectable in normal adult tissues. Angiogenesis and tissue repair are also hallmarks of advanced plaques. For imaging atherosclerotic plaques, the human antibody L19 (specific against ED-B) and a negative control antibody were labeled with radioiodine or infrared fluorophores and injected intravenously into atherosclerotic apolipoprotein E–null (ApoE) or normal wild-type mice. Aortas isolated 4 hours, 24 hours, and 3 days after injection exhibited a selective and stable uptake of L19 when using radiographic or fluorescent imaging. L19 binding was confined to the plaques as assessed by fat staining. Comparisons between fat staining and autoradiographies 24 hours after I-labeled L19 revealed a significant correlation (r=0.89; P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.0000150373.15149.ff