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Physiological Changes in Green Stems of Vitis vinifera L. cv. Chardonnay in Response to Esca Proper and Apoplexy Revealed by Proteomic and Transcriptomic Analyses
Among grapevine trunk diseases, esca proper and apoplexy commonly represent a threat for viticulture worldwide. To retrieve further information about the mechanisms activated in apoplectic and esca proper-affected plants, a two-dimensional gel electrophoresis (2-DE) based analysis was conducted on g...
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| Published in: | Journal of proteome research 2012-01, Vol.11 (1), p.461-475 |
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| creator | Spagnolo, Alessandro Magnin-Robert, Maryline Alayi, Tchilabalo Dilezitoko Cilindre, Clara Mercier, Laurence Schaeffer-Reiss, Christine Van Dorsselaer, Alain Clément, Christophe Fontaine, Florence |
| description | Among grapevine trunk diseases, esca proper and apoplexy commonly represent a threat for viticulture worldwide. To retrieve further information about the mechanisms activated in apoplectic and esca proper-affected plants, a two-dimensional gel electrophoresis (2-DE) based analysis was conducted on green stems from 26-year-old standing vines. Symptomatic and asymptomatic stems from both apoplectic (A) and esca proper-affected (E) plants compared to control (without visual symptom since 10 years) stems were studied. Thirty-three differentially expressed proteins were identified by nanoLC–MS/MS and included into three groups conceptually defined as proteins involved in (i) metabolism and energy, (ii) stress tolerance, and (iii) defense response. For nine of them, expression of the relative mRNA’s was also monitored by qRT-PCR. Proteome variations were specifically related to apoplexy and esca proper but were more similar in asymptomatic stems than in the symptomatic ones. Remarkable quantitative differences were noted for several proteins in symptomatic stems according to the expressed form, A and E. Results further indicate that similar responses are likely activated in asymptomatic stems but a various quantitative expression is triggered upon onset of apoplexy or esca proper symptoms while both kind of plants are infected by the same pathogenic fungi. |
| doi_str_mv | 10.1021/pr200892g |
| format | article |
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Chardonnay in Response to Esca Proper and Apoplexy Revealed by Proteomic and Transcriptomic Analyses</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)</source><creator>Spagnolo, Alessandro ; Magnin-Robert, Maryline ; Alayi, Tchilabalo Dilezitoko ; Cilindre, Clara ; Mercier, Laurence ; Schaeffer-Reiss, Christine ; Van Dorsselaer, Alain ; Clément, Christophe ; Fontaine, Florence</creator><creatorcontrib>Spagnolo, Alessandro ; Magnin-Robert, Maryline ; Alayi, Tchilabalo Dilezitoko ; Cilindre, Clara ; Mercier, Laurence ; Schaeffer-Reiss, Christine ; Van Dorsselaer, Alain ; Clément, Christophe ; Fontaine, Florence</creatorcontrib><description>Among grapevine trunk diseases, esca proper and apoplexy commonly represent a threat for viticulture worldwide. To retrieve further information about the mechanisms activated in apoplectic and esca proper-affected plants, a two-dimensional gel electrophoresis (2-DE) based analysis was conducted on green stems from 26-year-old standing vines. Symptomatic and asymptomatic stems from both apoplectic (A) and esca proper-affected (E) plants compared to control (without visual symptom since 10 years) stems were studied. Thirty-three differentially expressed proteins were identified by nanoLC–MS/MS and included into three groups conceptually defined as proteins involved in (i) metabolism and energy, (ii) stress tolerance, and (iii) defense response. For nine of them, expression of the relative mRNA’s was also monitored by qRT-PCR. Proteome variations were specifically related to apoplexy and esca proper but were more similar in asymptomatic stems than in the symptomatic ones. Remarkable quantitative differences were noted for several proteins in symptomatic stems according to the expressed form, A and E. Results further indicate that similar responses are likely activated in asymptomatic stems but a various quantitative expression is triggered upon onset of apoplexy or esca proper symptoms while both kind of plants are infected by the same pathogenic fungi.</description><identifier>ISSN: 1535-3893</identifier><identifier>EISSN: 1535-3907</identifier><identifier>DOI: 10.1021/pr200892g</identifier><identifier>PMID: 22050466</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Electrophoresis, Gel, Two-Dimensional ; Energy Metabolism - genetics ; Fungi - isolation & purification ; Gene Expression Profiling ; Life Sciences ; Peptide Fragments - chemistry ; Peptide Mapping ; Plant Diseases - microbiology ; Plant Immunity - genetics ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Stems - genetics ; Plant Stems - metabolism ; Plant Stems - physiology ; Proteolysis ; Proteome - chemistry ; Proteome - genetics ; Proteome - metabolism ; Proteomics ; Real-Time Polymerase Chain Reaction ; Stress, Physiological ; Tandem Mass Spectrometry ; Transcriptome ; Vitis - genetics ; Vitis - metabolism ; Vitis - physiology</subject><ispartof>Journal of proteome research, 2012-01, Vol.11 (1), p.461-475</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a348t-5ff5ff3edc46102e1179a38b06a8aaf26d7d766dbfbd0f335f79bfd363e1f0793</citedby><cites>FETCH-LOGICAL-a348t-5ff5ff3edc46102e1179a38b06a8aaf26d7d766dbfbd0f335f79bfd363e1f0793</cites><orcidid>0000-0001-8773-7926 ; 0000-0003-2918-8812 ; 0000-0003-0639-9078</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22050466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03624006$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Spagnolo, Alessandro</creatorcontrib><creatorcontrib>Magnin-Robert, Maryline</creatorcontrib><creatorcontrib>Alayi, Tchilabalo Dilezitoko</creatorcontrib><creatorcontrib>Cilindre, Clara</creatorcontrib><creatorcontrib>Mercier, Laurence</creatorcontrib><creatorcontrib>Schaeffer-Reiss, Christine</creatorcontrib><creatorcontrib>Van Dorsselaer, Alain</creatorcontrib><creatorcontrib>Clément, Christophe</creatorcontrib><creatorcontrib>Fontaine, Florence</creatorcontrib><title>Physiological Changes in Green Stems of Vitis vinifera L. cv. 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For nine of them, expression of the relative mRNA’s was also monitored by qRT-PCR. Proteome variations were specifically related to apoplexy and esca proper but were more similar in asymptomatic stems than in the symptomatic ones. Remarkable quantitative differences were noted for several proteins in symptomatic stems according to the expressed form, A and E. 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Chardonnay in Response to Esca Proper and Apoplexy Revealed by Proteomic and Transcriptomic Analyses</title><author>Spagnolo, Alessandro ; Magnin-Robert, Maryline ; Alayi, Tchilabalo Dilezitoko ; Cilindre, Clara ; Mercier, Laurence ; Schaeffer-Reiss, Christine ; Van Dorsselaer, Alain ; Clément, Christophe ; Fontaine, Florence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a348t-5ff5ff3edc46102e1179a38b06a8aaf26d7d766dbfbd0f335f79bfd363e1f0793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Energy Metabolism - genetics</topic><topic>Fungi - isolation & purification</topic><topic>Gene Expression Profiling</topic><topic>Life Sciences</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Mapping</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Immunity - genetics</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Stems - genetics</topic><topic>Plant Stems - metabolism</topic><topic>Plant Stems - physiology</topic><topic>Proteolysis</topic><topic>Proteome - chemistry</topic><topic>Proteome - genetics</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Stress, Physiological</topic><topic>Tandem Mass Spectrometry</topic><topic>Transcriptome</topic><topic>Vitis - genetics</topic><topic>Vitis - metabolism</topic><topic>Vitis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spagnolo, Alessandro</creatorcontrib><creatorcontrib>Magnin-Robert, Maryline</creatorcontrib><creatorcontrib>Alayi, Tchilabalo Dilezitoko</creatorcontrib><creatorcontrib>Cilindre, Clara</creatorcontrib><creatorcontrib>Mercier, Laurence</creatorcontrib><creatorcontrib>Schaeffer-Reiss, Christine</creatorcontrib><creatorcontrib>Van Dorsselaer, Alain</creatorcontrib><creatorcontrib>Clément, Christophe</creatorcontrib><creatorcontrib>Fontaine, Florence</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of proteome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spagnolo, Alessandro</au><au>Magnin-Robert, Maryline</au><au>Alayi, Tchilabalo Dilezitoko</au><au>Cilindre, Clara</au><au>Mercier, Laurence</au><au>Schaeffer-Reiss, Christine</au><au>Van Dorsselaer, Alain</au><au>Clément, Christophe</au><au>Fontaine, Florence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological Changes in Green Stems of Vitis vinifera L. cv. Chardonnay in Response to Esca Proper and Apoplexy Revealed by Proteomic and Transcriptomic Analyses</atitle><jtitle>Journal of proteome research</jtitle><addtitle>J. Proteome Res</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>11</volume><issue>1</issue><spage>461</spage><epage>475</epage><pages>461-475</pages><issn>1535-3893</issn><eissn>1535-3907</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Among grapevine trunk diseases, esca proper and apoplexy commonly represent a threat for viticulture worldwide. To retrieve further information about the mechanisms activated in apoplectic and esca proper-affected plants, a two-dimensional gel electrophoresis (2-DE) based analysis was conducted on green stems from 26-year-old standing vines. Symptomatic and asymptomatic stems from both apoplectic (A) and esca proper-affected (E) plants compared to control (without visual symptom since 10 years) stems were studied. Thirty-three differentially expressed proteins were identified by nanoLC–MS/MS and included into three groups conceptually defined as proteins involved in (i) metabolism and energy, (ii) stress tolerance, and (iii) defense response. For nine of them, expression of the relative mRNA’s was also monitored by qRT-PCR. Proteome variations were specifically related to apoplexy and esca proper but were more similar in asymptomatic stems than in the symptomatic ones. Remarkable quantitative differences were noted for several proteins in symptomatic stems according to the expressed form, A and E. Results further indicate that similar responses are likely activated in asymptomatic stems but a various quantitative expression is triggered upon onset of apoplexy or esca proper symptoms while both kind of plants are infected by the same pathogenic fungi.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>22050466</pmid><doi>10.1021/pr200892g</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-8773-7926</orcidid><orcidid>https://orcid.org/0000-0003-2918-8812</orcidid><orcidid>https://orcid.org/0000-0003-0639-9078</orcidid></addata></record> |
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| ispartof | Journal of proteome research, 2012-01, Vol.11 (1), p.461-475 |
| issn | 1535-3893 1535-3907 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03624006v1 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Electrophoresis, Gel, Two-Dimensional Energy Metabolism - genetics Fungi - isolation & purification Gene Expression Profiling Life Sciences Peptide Fragments - chemistry Peptide Mapping Plant Diseases - microbiology Plant Immunity - genetics Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plant Stems - genetics Plant Stems - metabolism Plant Stems - physiology Proteolysis Proteome - chemistry Proteome - genetics Proteome - metabolism Proteomics Real-Time Polymerase Chain Reaction Stress, Physiological Tandem Mass Spectrometry Transcriptome Vitis - genetics Vitis - metabolism Vitis - physiology |
| title | Physiological Changes in Green Stems of Vitis vinifera L. cv. Chardonnay in Response to Esca Proper and Apoplexy Revealed by Proteomic and Transcriptomic Analyses |
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