Physiological Changes in Green Stems of Vitis vinifera L. cv. Chardonnay in Response to Esca Proper and Apoplexy Revealed by Proteomic and Transcriptomic Analyses

Among grapevine trunk diseases, esca proper and apoplexy commonly represent a threat for viticulture worldwide. To retrieve further information about the mechanisms activated in apoplectic and esca proper-affected plants, a two-dimensional gel electrophoresis (2-DE) based analysis was conducted on g...

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Bibliographic Details
Published in:Journal of proteome research 2012-01, Vol.11 (1), p.461-475
Main Authors: Spagnolo, Alessandro, Magnin-Robert, Maryline, Alayi, Tchilabalo Dilezitoko, Cilindre, Clara, Mercier, Laurence, Schaeffer-Reiss, Christine, Van Dorsselaer, Alain, Clément, Christophe, Fontaine, Florence
Format: Article
Language:English
Subjects:
Electrophoresis, Gel, Two-Dimensional
Energy Metabolism - genetics
Fungi - isolation & purification
Gene Expression Profiling
Life Sciences
Peptide Fragments - chemistry
Peptide Mapping
Plant Diseases - microbiology
Plant Immunity - genetics
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Stems - genetics
Plant Stems - metabolism
Plant Stems - physiology
Proteolysis
Proteome - chemistry
Proteome - genetics
Proteome - metabolism
Proteomics
Real-Time Polymerase Chain Reaction
Stress, Physiological
Tandem Mass Spectrometry
Transcriptome
Vitis - genetics
Vitis - metabolism
Vitis - physiology
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Summary:Among grapevine trunk diseases, esca proper and apoplexy commonly represent a threat for viticulture worldwide. To retrieve further information about the mechanisms activated in apoplectic and esca proper-affected plants, a two-dimensional gel electrophoresis (2-DE) based analysis was conducted on green stems from 26-year-old standing vines. Symptomatic and asymptomatic stems from both apoplectic (A) and esca proper-affected (E) plants compared to control (without visual symptom since 10 years) stems were studied. Thirty-three differentially expressed proteins were identified by nanoLC–MS/MS and included into three groups conceptually defined as proteins involved in (i) metabolism and energy, (ii) stress tolerance, and (iii) defense response. For nine of them, expression of the relative mRNA’s was also monitored by qRT-PCR. Proteome variations were specifically related to apoplexy and esca proper but were more similar in asymptomatic stems than in the symptomatic ones. Remarkable quantitative differences were noted for several proteins in symptomatic stems according to the expressed form, A and E. Results further indicate that similar responses are likely activated in asymptomatic stems but a various quantitative expression is triggered upon onset of apoplexy or esca proper symptoms while both kind of plants are infected by the same pathogenic fungi.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr200892g