Impact of alcohol and cigarette smoking consumption in male fertility potential: Looks at lipid peroxidation, enzymatic antioxidant activities and sperm DNA damage

Summary Alcohol intake and cigarette smoking are the major lifestyle factors with negative impact on fertility. We were interested to evaluate the negative impact of these factors on oxidative stress (OS), enzymatic antioxidant activity (EAO) of spermatozoa and on its DNA damage. This study included...

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Bibliographic Details
Published in:Andrologia 2018-04, Vol.50 (3), p.e12926-n/a
Main Authors: Aboulmaouahib, S., Madkour, A., Kaarouch, I., Sefrioui, O., Saadani, B., Copin, H., Benkhalifa, M., Louanjli, N., Cadi, R.
Format: Article
Language:English
Subjects:
alcohol consumption
Alcohol use
Alcoholic beverages
Aniline
Antioxidants
Catalase
Chromatin
Cigarette smoking
Deoxyribonucleic acid
DNA
DNA damage
DNA fragmentation
enzymatic antioxidant activity
Glutathione reductase
infertile men
Infertility
Life Sciences
Lipid peroxidation
Oxidative stress
Reproductive technologies
Smoking
Sperm
sperm DNA damage
Superoxide dismutase
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Summary:Summary Alcohol intake and cigarette smoking are the major lifestyle factors with negative impact on fertility. We were interested to evaluate the negative impact of these factors on oxidative stress (OS), enzymatic antioxidant activity (EAO) of spermatozoa and on its DNA damage. This study included 108 male infertile patients with normal range of sperm conventional parameters but with unexplained infertility in assisted reproductive technologies programme. Firstly, OS was analysed based on lipid peroxidation (MDA) and EAO which included catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). Secondly, we evaluated DNA fragmentation by TUNEL assay and chromatin decondensation by aniline blue colouration. The whole lot was divided into four groups: control (nonalcoholic and nonsmoker patients), alcohol group, smoking group and alcohol‐smoking group. The results showed, in three last groups compared to control an increased CAT, SOD and GR activities with high MDA level especially in smoking and alcohol‐smoking group. The latter showed the highest values of DNA fragmentation and chromatin decondensation (31% and 39%) to exceed DNA damage normal range. Indeed, smoking and alcohol intake lead to increase EAO due to long‐term unbalanced antioxidant/oxidation ratio with high OS which cause consequently sperm DNA damage calling in need by urgency to change the lifestyle behaviour.
ISSN:0303-4569
1439-0272
DOI:10.1111/and.12926