Combination of in vitro thermally-accelerated ageing and Fourier-Transform Infrared spectroscopy to predict scaffold lifetime

•FTIR spectroscopy to simply and straightforwardly screen scaffold durability•Investigation of scaffold degradation using thermally-accelerated ageing•Arrhenius extrapolation and activation energy to predict scaffold lifetime at 37°C Biodegradable elastomers face a growing use in soft tissue enginee...

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Bibliographic Details
Published in:Polymer degradation and stability 2021-01, Vol.183, p.109454, Article 109454
Main Authors: Langueh, Credson, Changotade, Sylvie, Ramtani, Salah, Lutomski, Didier, Rohman, Géraldine
Format: Article
Language:English
Subjects:
Accelerated ageing
Accelerated aging tests
Accelerated tests
Activation energy
Biodegradability
Biodegradable materials
Biodegradation
Chemical composition
Chemical Sciences
Compression tests
Degradation
Durability
Elastomers
Fourier transforms
FTIR spectroscopy
Gravimetry
Infrared analysis
Infrared spectroscopy
Lifetime
Material properties
Mechanical properties
Scaffold
Scaffolds
Soft tissues
Spectrum analysis
Strain
Thermomechanical properties
Thermomechanical treatment
Tissue engineering
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Summary:•FTIR spectroscopy to simply and straightforwardly screen scaffold durability•Investigation of scaffold degradation using thermally-accelerated ageing•Arrhenius extrapolation and activation energy to predict scaffold lifetime at 37°C Biodegradable elastomers face a growing use in soft tissue engineering due to the possibility to tune, by an appropriate selection of the synthesis and process conditions, the material thermo-mechanical properties to match the stress–strain behavior of the tissue to replace. However, changes in material properties can impact drastically the scaffold durability and therefore the efficiency of tissue reconstruction. Few studies focus on approaches allowing the prediction of the scaffold lifetime, while there is a need for strategies using accelerated testing protocols and versatile tools to easily investigate on the material degradation rate. In the present study, elastomeric cross-linked poly(ester-urethane-urea) scaffolds have been developed through an emulsion technique allowing to produce highly interconnected porous structure. Thermally-accelerated ageing was performed in cell culture medium at different temperatures: 37°C, 55°C, 75°C and 90°C. The degradation process was followed by gravimetry, swelling measurements, compression tests and infrared spectroscopy. The study revealed that the scaffold chemical composition variation was temperature dependant and its analysis by Fourier-Transform infrared spectroscopy allowed an easy determination of the activation energy of the hydrolytic degradation process, leading to the prediction of the scaffold lifetime at 37°C using Arrhenius extrapolation. This approach could be used to simply and straightforwardly screen the durability of new scaffolds.
ISSN:0141-3910
1873-2321
DOI:10.1016/j.polymdegradstab.2020.109454