Isolation and in vitro antibacterial activity of astilbin, the bioactive flavanone from the leaves of Harungana madagascariensis Lam. ex Poir. ( Hypericaceae)

Harungana madagascariensis is well known for its topical antibacterial properties used in the elaboration of a lot of skin hygiene products. The aim of this study was, on the one hand, to evaluate the in vitro antibacterial activities of aqueous, ethanolic and ethyl acetate crude extracts of Harunga...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2006-06, Vol.106 (2), p.272-278
Main Authors: Moulari, B., Pellequer, Y., Lboutounne, H., Girard, C., Chaumont, J.-P., Millet, J., Muyard, F.
Format: Article
Language:English
Subjects:
Acetates
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - isolation & purification
Anti-Bacterial Agents - pharmacology
Antibacterial activity
antibacterial properties
Astilbin
Bioautography
Biological and medical sciences
chemical constituents of plants
Chemical Sciences
chemical structure
Clusiaceae - chemistry
Corynebacterium - drug effects
Deodorants - pharmacology
flavanones
Flavonols - chemistry
Flavonols - isolation & purification
Flavonols - pharmacology
General pharmacology
Harungana madagascariensis
Harungana madagascariensis leaf extracts
leaves
Life Sciences
Medical sciences
medicinal plants
medicinal properties
Microbial Sensitivity Tests
Micrococcus - drug effects
Minimal bactericidal concentration
Minimal inhibitory concentration
Minimal inhibitory quantity
Pharmaceutical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
plant extracts
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Leaves
skin
Solvents
Staphylococcus - drug effects
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Summary:Harungana madagascariensis is well known for its topical antibacterial properties used in the elaboration of a lot of skin hygiene products. The aim of this study was, on the one hand, to evaluate the in vitro antibacterial activities of aqueous, ethanolic and ethyl acetate crude extracts of Harungana madagascariensis leaves against bacterial strains representative of skin microflora and, on the other hand, to determine the chemical structure of the active compound. Only the ethyl acetate leaf extract presented important antibacterial activity. Its fractionation was carried out by column chromatography using silica gel 60 and it yielded 11 fractions. A bioautographic method, revealed in these fractions the presence of a flavanone as the active compound astilbin or 3- O-α- l-rhamnoside-5,7,3′,4′-tetrahydroxydihydroflavonol which was identified on the basis of its spectroscopic data. Concerning the antibacterial activity against the representative skin microflora of the armpit and feet, MIC and MBC ranged from 25 to 250 and 100 to 750 μg ml −1, respectively. The results showed that some bacteria considered to be responsible for bad odours at the armpit and feet levels, were destroyed at 200 μg ml −1 (MBC), a concentration sparing most of the useful saprophytic microflora. The minimal inhibitory quantity (MIQs) of astilbin ranged from 50 to 100 μg.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2006.01.008