The microbial community associated with pea seeds (Pisum sativum) of different geographical origins

Aims The pea seeds ( Pisum sativum ) hosts a complex microbial community that is exposed to environmental constraints modulated by biotic, abiotic and anthropogenic factors. The aim of this study was to explore the effect of geographical origins on the composition of the microbial communities associ...

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Bibliographic Details
Published in:Plant and soil 2021-05, Vol.462 (1-2), p.405-427
Main Authors: Chartrel, Valentine, Dugat-Bony, Eric, Sarthou, Anne-Sophie, Huchette, Sophie, Bonnarme, Pascal, Irlinger, Françoise
Format: Article
Language:English
Subjects:
Anthropogenic factors
Biomedical and Life Sciences
Climate
Composition
Dominant species
Ecology
Environmental aspects
Food industry
Food processing
Geographical distribution
Life Sciences
Microbial activity
Microbial colonies
Microbiomes
Microbiota
Microorganisms
Next-generation sequencing
Origins
Peas
Phylogeny
Pisum sativum
Plant Physiology
Plant Sciences
Proteins
Regular Article
rRNA 16S
Seeds
Soil Science & Conservation
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Summary:Aims The pea seeds ( Pisum sativum ) hosts a complex microbial community that is exposed to environmental constraints modulated by biotic, abiotic and anthropogenic factors. The aim of this study was to explore the effect of geographical origins on the composition of the microbial communities associated with pea seeds from storage locations in different countries. These seeds are used by Roquette Frères® as source proteins for human food after industrial extraction process of their proteins. Methods The high-throughput sequencing of two phylogenetic markers (V3-V4 regions of the 16S rRNA gene and ITS2 region) was used to evaluate the structure of the microbial community associated with pea seeds. The pea seeds were harvested by different suppliers from three countries (France, Sweden and Canada) with different climates and stored in six cooperatives. Representative microbial strains of dominant species were isolated and characterized using phenotypic methods. Results Although the pea seed microbiota shared a dominant core microbiota at the genus level, seeds showed a species-specific, highly unique microbial signature with variable abundances according to origin. Origin was identified as the main driver of the composition of the pea seed microbiota. Conclusions These results provide an initial insight into the broad factors such as country and cooperative that may determine the assembly of the pea microbiota before processing.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-021-04856-6