Carnosine, oxidative and carbonyl stress, antioxidants, and muscle fiber characteristics of quadriceps muscle of patients with COPD

Carnosine, particularly present in fast-twitch fibers, was investigated in the quadriceps of patients with chronic obstructive pulmonary disease (COPD). Carnosine concentration was similar between patients with COPD and healthy controls but was 30% lower in patients with severe/very severe COPD, des...

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Published in:Journal of applied physiology (1985) 2021-10, Vol.131 (4), p.1230-1240
Main Authors: De Brandt, J., Burtin, C., Pomiès, P., Vandenabeele, F., Verboven, K., Aumann, J., Blancquaert, L., Everaert, I., Van Ryckeghem, L., Cops, J., Hayot, M., Spruit, M. A., Derave, W.
Format: Article
Language:English
Subjects:
Abnormalities
Air flow
Antioxidants
Biopsy
Carbonyl compounds
Carbonyls
Carnosine
Chronic obstructive pulmonary disease
Fibers
Human health and pathology
Life Sciences
Lung diseases
Muscles
Obstructive lung disease
Physical activity
Quadriceps muscle
Respiratory function
Skeletal muscle
Stress
Tissues and Organs
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Summary:Carnosine, particularly present in fast-twitch fibers, was investigated in the quadriceps of patients with chronic obstructive pulmonary disease (COPD). Carnosine concentration was similar between patients with COPD and healthy controls but was 30% lower in patients with severe/very severe COPD, despite their high proportion of fast-twitch fibers, versus patients with mild/moderate COPD. As no oxidative/carbonyl stress markers or antioxidants were affected, the observed carnosine deficiency is thought to be a possible first sign of muscle redox balance abnormalities. Oxidative/carbonyl stress is elevated in lower-limb muscles of patients with chronic obstructive pulmonary disease (COPD). Carnosine is a skeletal muscle antioxidant particularly present in fast-twitch fibers. The aims of the present study were to compare muscle carnosine, oxidative/carbonyl stress, antioxidants, and fiber characteristics between patients with COPD and healthy controls (HCs) and between patients after stratification for airflow limitation (mild/moderate vs. severe/very severe), as well as to investigate correlates of carnosine in patients with COPD. A vastus lateralis muscle biopsy was obtained from 40 patients with stable COPD and 20 age- and sex-matched HCs. Carnosine, oxidative/carbonyl stress, antioxidants, fiber characteristics, quadriceps strength and endurance (QE), V̇o 2 peak (incremental cycle test), and physical activity (PA) were determined. Patients with COPD had a similar carnosine concentration [4.16 mmol/kg wet weight (WW; SD = 1.93)] to HCs [4.64 mmol/kg WW (SD = 1.71)] and significantly higher percentage of fast-twitch fibers and lower QE, V̇o 2 peak, and PA versus HCs. Patients with severe/very severe COPD had a 31% lower carnosine concentration [3.24 mmol/kg WW (SD = 1.79); n = 15] versus patients with mild/moderate COPD [4.71 mmol/kg WW (SD = 1.83); n = 25; P = 0.02] and significantly lower V̇o 2 peak and PA versus patients with mild/moderate COPD. Carnosine correlated significantly with QE ( r s = 0.427), V̇o 2 peak ( r s = 0.334), PA ( r s = 0.379), and lung function parameters in patients with COPD. In conclusion, despite having the highest proportion of fast-twitch fibers, patients with severe/very severe COPD displayed a 31% lower muscle carnosine concentration compared with patients with mild/moderate COPD. As no other markers of oxidative/carbonyl stress or antioxidants were affected, the observed carnosine deficiency is thought to be a possible firs
ISSN:8750-7587
1522-1601
DOI:10.1152/japplphysiol.00200.2021