Nanocrystal-Encoded Fluorescent Microbeads for Proteomics:  Antibody Profiling and Diagnostics of Autoimmune Diseases

The first application of nanocrystal (NC)-encoded microbeads to clinical proteomics is demonstrated by multiplexed detection of circulating autoantibodies, markers of systemic sclerosis. Two-color complexes, consisting of NC-encoded, antigen-covered beads, anti-antigen antibody or clinical serum sam...

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Bibliographic Details
Published in:Nano letters 2007-08, Vol.7 (8), p.2322-2327
Main Authors: Sukhanova, Alyona, Susha, Andrei S, Bek, Alpan, Mayilo, Sergiy, Rogach, Andrey L, Feldmann, Jochen, Oleinikov, Vladimir, Reveil, Brigitte, Donvito, Beatrice, Cohen, Jacques H. M, Nabiev, Igor
Format: Article
Language:English
Subjects:
Antibodies - analysis
Antibodies - immunology
Autoimmune Diseases - diagnosis
Autoimmune Diseases - immunology
Biological and medical sciences
Biotechnology
Cross-disciplinary physics: materials science
rheology
Engineering Sciences
Exact sciences and technology
Fluorescence Resonance Energy Transfer - methods
Fluorescent Antibody Technique - methods
Fundamental and applied biological sciences. Psychology
Human health and pathology
Humans
Immunoassay - methods
Life Sciences
Materials science
Methods. Procedures. Technologies
Micro and nanotechnologies
Microelectronics
Microspheres
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Nanostructures - chemistry
Nanostructures - ultrastructure
Others
Physics
Proteomics - methods
Various methods and equipments
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Summary:The first application of nanocrystal (NC)-encoded microbeads to clinical proteomics is demonstrated by multiplexed detection of circulating autoantibodies, markers of systemic sclerosis. Two-color complexes, consisting of NC-encoded, antigen-covered beads, anti-antigen antibody or clinical serum samples, and dye-tagged detecting antibodies, were observed using flow cytometry assays and on the surface of single beads. The results of flow cytometry assays correlated with the ELISA technique and provided clear discrimination between the sera samples of healthy donors and patients with autoimmune disease. Microbead fluorescence signals exhibited narrow distribution regardless of their surface antigen staining, without the need of any fluorescence compensationa parameter determining the limit of sensitivity of flow cytometry assays. In single bead measurements, less than 30 dye-labeled antibodies interacting with the topoI-specific antibodies at the surface of a bead have been detected by the emission of dye excited through the FRET from NCs. In this format, the antibody−bead interaction reaction turns specifically the fluorescence signal from dye label off and on, additionally increasing autoantibody detection sensitivity.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl070966+