Resonance Energy Transfer Improves the Biological Function of Bacteriorhodopsin within a Hybrid Material Built from Purple Membranes and Semiconductor Quantum Dots

Purple membrane (PM) from bacteria Halobacterium salinarum contains a photochromic protein bacteriorhodopsin (bR) arranged in a 2D hexagonal nanocrystalline lattice (Figure ). Absorption of light by the protein-bound chromophore retinal results in pumping the protons through the PM creating an elect...

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Bibliographic Details
Published in:Nano letters 2010-07, Vol.10 (7), p.2640-2648
Main Authors: Rakovich, Aliaksandra, Sukhanova, Alyona, Bouchonville, Nicolas, Lukashev, Evgeniy, Oleinikov, Vladimir, Artemyev, Mikhail, Lesnyak, Vladimir, Gaponik, Nikolai, Molinari, Michael, Troyon, Michel, Rakovich, Yury P, Donegan, John F, Nabiev, Igor
Format: Article
Language:eng ; rus
Subjects:
Archaeal Proteins - chemistry
Bacteriorhodopsins - chemistry
Bioengineering
Engineering Sciences
Fluorescence Resonance Energy Transfer - methods
Halobacterium salinarum - chemistry
Life Sciences
Micro and nanotechnologies
Microelectronics
Purple Membrane - chemistry
Quantum Dots
Semiconductors
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Summary:Purple membrane (PM) from bacteria Halobacterium salinarum contains a photochromic protein bacteriorhodopsin (bR) arranged in a 2D hexagonal nanocrystalline lattice (Figure ). Absorption of light by the protein-bound chromophore retinal results in pumping the protons through the PM creating an electrochemical gradient which is then used by the ATPases to energize the cellular processes. Energy conversion, photochromism, and photoelectrism are the inherent effects which are employed in many bR technical applications. , bR, along with the other photosensitive proteins, is not able to deal with the excess energy of photons in UV and blue spectral region and utilizes less than 0.5% of the energy from the available incident solar light for its biological function. Here, we proceed with optimization of bR functions through the engineering of a “nanoconverter” of solar energy based on semiconductor quantum dots (QDs) tagged with the PM. These nanoconverters are able to harvest light from deep-UV to the visible region and to transfer this additionally collected energy to bR via Förster resonance energy transfer (FRET). We show that specific nanobio-optical and spatial coupling of QDs (donor) and bR retinal (acceptor) provide a means to achieve FRET with efficiency approaching 100%. We have finally demonstrated that the integration of QDs within PM significantly increases the efficiency of light-driven transmembrane proton pumping, which is the main bR biological function. This new QD−PM hybrid material will have numerous optoelectronic, photonic, and photovoltaic applications based on its energy conversion, photochromism, and photoelectrism properties.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl1013772