Analysis of inspiratory and expiratory muscles using ultrasound in rats: A reproducible and non-invasive tool to study respiratory function

•First study in rats to report data about respiratory muscles using ultrasounds.•High accuracy and reproducibility to evaluate respiratory muscles in rats by ultrasounds.•Validation of ultrasound results by post-mortem histological analyses.•Correlation between tidal volume and rectus abdominis area...

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Bibliographic Details
Published in:Respiratory physiology & neurobiology 2021-03, Vol.285, p.103596-103596, Article 103596
Main Authors: Fayssoil, Abdallah, Michel-Flutot, Pauline, Lofaso, Frédéric, Carlier, Robert, El Hajjam, Mostafa, Vinit, Stéphane, Mansart, Arnaud
Format: Article
Language:English
Subjects:
Diaphragm
Life Sciences
Neurons and Cognition
Rat
Rectus abdominis
Ultrasound
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Summary:•First study in rats to report data about respiratory muscles using ultrasounds.•High accuracy and reproducibility to evaluate respiratory muscles in rats by ultrasounds.•Validation of ultrasound results by post-mortem histological analyses.•Correlation between tidal volume and rectus abdominis area. Ultrasound imaging is a non-invasive technique to assess organ function. Its potential application in rodents to evaluate respiratory function remains poorly investigated. We aimed to assess and validate ultrasound technique in rats to analyze inspiratory and expiratory muscles. We measured respiratory parameters to provide normal eupneic values. Histological studies and plethysmography were used to validate the technique and assess the physiological implications. A linear relationship was observed between ultrasound and histological data for diaphragm and rectus abdominis (RA) measurement. The tidal volume was significantly correlated with the right + left RA area (r = 0.76, p < 0.001), and the rapid shallow breathing index was significantly and inversely correlated with the right + left RA area (r=-0.53, p < 0.05). In the supine position, the right and left diaphragm expiratory thickness were not associated with tidal volume obtained in the physiological position. Ultrasound imaging is highly accurate and reproducible to assess and follow up diaphragm and RA structure and function in rats.
ISSN:1569-9048
1878-1519
DOI:10.1016/j.resp.2020.103596