The Arabidopsis Cytosolic Thioredoxin h 5 Gene Induction by Oxidative Stress and Its W-Box-Mediated Response to Pathogen Elicitor

Abstract The AtTRXh5 protein belongs to the cytosolic thioredoxins h family that, in Arabidopsis, contains eight members showing very distinct patterns and levels of expression. Here, we show that the AtTRXh5 gene is up-regulated during wounding, abscission, and senescence, as well as during incompa...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2004-03, Vol.134 (3), p.1006-1016
Main Authors: Laloi, Christophe, Mestres-Ortega, Dominique, Marco, Yves, Meyer, Yves, Reichheld, Jean-Philippe
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Genomics
Life Sciences
Molecular biology
Plant breeding
Vegetal Biology
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Summary:Abstract The AtTRXh5 protein belongs to the cytosolic thioredoxins h family that, in Arabidopsis, contains eight members showing very distinct patterns and levels of expression. Here, we show that the AtTRXh5 gene is up-regulated during wounding, abscission, and senescence, as well as during incompatible interactions with the bacterial pathogen Pseudomonas syringae. By electrophoretic mobility shift assays, a binding activity on a W-box in the AtTRXh5 promoter region was found induced by treatments with the P. syringae-derived elicitor peptide flg22, suggesting that a WRKY transcription factor controls AtTRXh5 induction upon elicitor treatment. Remarkably, AtTRXh5 was up-regulated in plants overexpressing WRKY6. More generally, AtTRXh5 is induced in response to oxidative stress conditions. Collectively, our data indicate a possible implication of the cytosolic thioredoxin AtTRXh5 in response to pathogens and to oxidative stresses. In addition, this regulation is unique to AtTRXh5 among the thioredoxin h family, arguing in favor of a speciation rather than to a redundancy of the members of this multigenic family.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.103.035782